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Anti-CDK1 antibody [E161] (ab32384)

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Overview

Product name

Anti-CDK1 antibody [E161]
See all CDK1 products (26) ...

Description

Rabbit monoclonal [E161] to CDK1

Specificity

The antibody detects Cdc2 without phosphorylation on Tyrosine 15. It does not detect Y15 phosphorylated Cdc2.

Tested applications

WB, IHC-P, ICC, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Cow

Immunogen

Synthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2.

Positive control

WB: HeLa cell lysate. IHC-P: Human lymphoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E161

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Neurology process >> Neural Signal Transduction
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

  • Western blot - Cdc2 antibody [E161] (ab32384)Western blot - Cdc2 antibody [E161] (ab32384) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Cdc2 antibody [E161] (ab32384)Immunohistochemistry (Paraffin-embedded sections) - Cdc2 antibody [E161] (ab32384) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/500.
IHC-P: 1/50.
IP: 1/50.
WB: 1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Tissue specificity

Isoform 2 is found in breast cancer tissues.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP06493 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdc 2 antibody
  • Cdc2 antibody
  • CDK 1 antibody
  • CDK1 antibody
  • CDK1_HUMAN antibody
  • Cell division control protein 2 antibody
  • Cell division control protein 2 homolog antibody
  • Cell division cycle 2 G1 to S and G2 to M antibody
  • Cell division protein kinase 1 antibody
  • Cell Divsion Cycle 2 Protein antibody
  • Cyclin Dependent Kinase 1 antibody
  • Cyclin-dependent kinase 1 antibody
  • DKFZp686L20222 antibody
  • MGC111195 antibody
  • p34 Cdk1 antibody
  • p34 Protein Kinase antibody
see all

Database links

see all

Anti-CDK1 antibody [E161] images:

  Western blot - Cdc2 antibody [E161] (ab32384)

Western blot - Cdc2 antibody [E161] (ab32384)

All lanes : Anti-CDK1 antibody [E161] (ab32384) at 1/5000 dilution

Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with hydroxyurea
Lane 3 : HeLa cell lysate, treated with phosphatase


Predicted band size : 34 kDa
Observed band size : 34 kDa

  Immunohistochemistry (Paraffin-embedded sections) - Cdc2 antibody [E161] (ab32384)

Immunohistochemistry (Paraffin-embedded sections) - Cdc2 antibody [E161] (ab32384)

Ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.

Protocols

  • Phosphatase treatment for WB with ab32384

    • References for Anti-CDK1 antibody [E161] (ab32384)

    ab32384 has not yet been referenced specifically in any publications.

    Publishing research using ab32384? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"