Anti-CDK1 antibody [E161] (ab32384)

Overview

• Product nameAnti-CDK1 antibody [E161]See all CDK1 primary antibodies ...
• Description
  Rabbit monoclonal [E161] to CDK1
• Tested applicationsWB, IHC-P, ICC, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Cow
• Immunogen

  Synthetic peptide corresponding to internal residues of Human CDK1.

• Positive controlWB: HeLa cell lysate. IHC-P: Human lymphoma.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• Clonality Monoclonal
• Clone numberE161
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
• Tissue specificityIsoform 2 is found in breast cancer tissues.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P06493 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281061 Cow
  • Entrez Gene: 983 Human
  • Entrez Gene: 983 Human
  • Entrez Gene: 983 Human
  • Entrez Gene: 12534 Mouse
  • Entrez Gene: 54237 Rat
  • Omim: 116940 Human
  • SwissProt: P48734 Cow

  • SwissProt: P06493 Human
  • SwissProt: P06493 Human
  • SwissProt: P06493 Human
  • SwissProt: P11440 Mouse
  • SwissProt: P39951 Rat
  • Unigene: 334562 Human
  • Unigene: 281367 Mouse
  • Unigene: 6934 Rat

  see all

• Alternative names
  Cdc 2 antibodyCdc2 antibodyCDC28A antibody

  CDK 1 antibodyCDK1 antibodyCDK1_HUMAN antibodyCDKN1 antibodyCELL CYCLE CONTROLLER CDC2 antibodyCell division control protein 2 antibodyCell division control protein 2 homolog antibodyCell division cycle 2 G1 to S and G2 to M antibodyCell division protein kinase 1 antibodyCell Divsion Cycle 2 Protein antibodyCyclin Dependent Kinase 1 antibodyCyclin-dependent kinase 1 antibodyDKFZp686L20222 antibodyMGC111195 antibodyp34 Cdk1 antibodyp34 Protein Kinase antibodyP34CDC2 antibody

  see all

Anti-CDK1 antibody [E161] images

• 

  Immunohistochemistry (Paraffin-embedded sections) - Cdc2 antibody [E161] (ab32384)
  Ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.
• 

  Western blot - Anti-CDK1 antibody [E161] (ab32384)
  All lanes : Anti-CDK1 antibody [E161] (ab32384) at 1/1000 dilution
  
  Lane 1 : A431 cell lysate
  Lane 2 : HepG2 cell lysate
  Lane 3 : SKBR3 cell lysate
  Lane 4 : HeLa cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 34 kDa
  

Protocols

• Phosphatase treatment for WB with ab32384