![Western blot - Cdc2 antibody [E53] (ab32030) image](#ab32030_1.jpg)
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- Secondary antibodies
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Product name
Anti-CDK1 antibody [E53]
See all CDK1 products (26) ...
Description
Rabbit monoclonal [E53] to CDK1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide (Human).
Positive control
WB: Hela cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Clonality
Monoclonal
Clone number
E53
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Neurology process >> Neural Signal Transduction
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Western blot - Cdc2 antibody [E53] (ab32030)
![Western blot - Cdc2 antibody [E53] (ab32030) image](/ps/datasheet/Images/32/ab32030/ab32030_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab32030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: 1/40.
WB: 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for FACS, ICC or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Tissue specificity
Isoform 2 is found in breast cancer tissues.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP06493
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 2 antibody
- Cdc2 antibody
- CDK 1 antibody
see all
Database links
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
Anti-CDK1 antibody [E53] images:
Western blot - Cdc2 antibody [E53] (ab32030)
Anti-CDK1 antibody [E53] (ab32030) at 1/5000 dilution + Hela cell lysate
Predicted band size : 34 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-CDK1 antibody [E53] (ab32030)
ab32030 has not yet been referenced specifically in any publications.
Publishing research using ab32030? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"