Anti-Cdc20 antibody (ab155921)
Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cdc20 antibody
See all Cdc20 primary antibodies -
Description
Rabbit polyclonal to Cdc20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 14-270 of Human Cdc20 (Uniprot ID: Q12834).
-
Positive control
- Recombinant Human Cdc20 protein (ab131769) can be used as a positive control in WB. HeLa whole cell lysate; Human breast carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
Target
-
Function
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats. -
Developmental stage
Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. -
Post-translational
modificationsPhosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. - Information by UniProt
-
Database links
- Entrez Gene: 991 Human
- Entrez Gene: 107995 Mouse
- Omim: 603618 Human
- SwissProt: Q12834 Human
- SwissProt: Q9JJ66 Mouse
- Unigene: 524947 Human
- Unigene: 289747 Mouse
-
Alternative names
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab155921 has been referenced in 1 publication.
- Liang LY & Li GS The Roles of KIFC1 in the Development of Osteosarcoma: Characterization of Potential Therapeutic Targets. Comput Math Methods Med 2022:5039134 (2022). PubMed: 35479190