Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

Product Name 

Cdc25A antibody [DCS-120 + DCS-121]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DCS-120 + DCS-121] to Cdc25A

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu, Rat

Specificity 

This antibody reacts with a 65 kD protein known as Cdc25A.

Tested applications 
(see key)

IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/50 - 1/100.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.
IHC: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method.
We suggest an incubation period of 30 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil.

Cellular localization 

Nuclear membrane

Research areas 

Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases

Relevance 

Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DCS-120 + DCS-121

Isotype 

IgG2a

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C.

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Cdc25A antibody [DCS-120 + DCS-121] images:

References for Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

  This product has been used in:

Leisser C et al. Subcellular localisation of Cdc25A determines cell fate. Cell Death Differ 11:134 (2004). PubMed: 14685169
If you publish research using ab2357 please let us know so that we can cite the reference on this datasheet.

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