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Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

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Overview

Product name

Anti-Cdc25A antibody [DCS-120 + DCS-121]
See all Cdc25A products (18) ...

Description

Mouse monoclonal [DCS-120 + DCS-121] to Cdc25A

Specificity

This antibody reacts with a 65 kD protein known as Cdc25A.

Tested applications

WB, IP, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Rat, Human

Immunogen

Recombinant full length protein (Human).

Positive control

Tonsil.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

DCS-120 + DCS-121

Myeloma

unknown

Isotype

IgG2a

Light chain type

unknown

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdc25A antibody [DCS-120 + DCS-121] (ab2357)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdc25A antibody [DCS-120 + DCS-121] (ab2357) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Sequence similarities

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Domain

The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.

Post-translational
modifications

Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.

Target information above from: UniProt accessionP30304 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdc 25a antibody
  • CDC25A antibody
  • CDC25A2 antibody
  • CDC25A2 CAG isoform antibody
  • Cell division cycle 25 homolog A (S. pombe) antibody
  • Cell division cycle 25A antibody
  • Cell division cycle 25A isoform a antibody
  • Cell division cycle 25A isoform b antibody
  • D9Ertd393e antibody
  • Dual specificity phosphatase CDC25A antibody
  • EC 3.1.3.48 antibody
  • M phase inducer phosphatase 1 antibody
  • M-phase inducer phosphatase 1 antibody
  • MGC115549 antibody
  • MPIP1_HUMAN antibody
see all

Anti-Cdc25A antibody [DCS-120 + DCS-121] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

ab2357 staining human tonsil by IHC-P.

References for Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

This product has been referenced in:

  • Sims JK & Wade PA Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin. Mol Biol Cell 22:3094-102 (2011).Read more (PubMed: 21737684) »
  • Kasahara Ket al. 14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage. EMBO J 29:2802-12 (2010). WB; Human.Read more (PubMed: 20639859) »

See all 3 publications for this product

Publishing research using ab2357? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"