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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Overview
Product name
Anti-Cdc25A antibody
See all Cdc25A products (18) ...
Description
Rabbit polyclonal to Cdc25A
Specificity
This antibody is specific for Cdc25A.
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthesized non-phosphopeptide derived from human Cdc25A around the phosphorylation site of serine 75.
Positive control
Extracts from A2780 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/500 - 1/1000.Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Domain
The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
Post-translational
modifications
Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
Target information above from: UniProt accessionP30304
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
see all
Database links
- Entrez Gene: 993 Human
- Entrez Gene: 12530 Mouse
- Entrez Gene: 171102 Rat
- Omim: 116947 Human
- SwissProt: P30304 Human
- SwissProt: P48964 Mouse
- SwissProt: Q80VD9 Mouse
- SwissProt: P48965 Rat
see all
Anti-Cdc25A antibody images:
Western blot - Cdc25A antibody (ab47400)
All lanes : Anti-Cdc25A antibody (ab47400) at 1/500 dilution
Lane 1 : Extracts from A2780 cells treated with UV
Lane 2 : Extracts from A2780 cells treated with UV and peptide
Predicted band size : 59 kDa
Observed band size : 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cdc25A antibody(ab47400)
Ab47400 staining human tonsil. Staining is localised to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Cdc25A antibody (ab47400)
ab47400 has not yet been referenced specifically in any publications.
Publishing research using ab47400? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"