You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab92892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 59 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionTyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
    • Sequence similaritiesBelongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • DomainThe phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
    • Post-translational
      modifications      Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.

      • Target information above from: UniProt accession P30304 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Cdc 25a antibodyCDC25A antibodyCDC25A2 antibody
          CDC25A2 CAG isoform antibodyCell division cycle 25 homolog A (S. pombe) antibodyCell division cycle 25A antibodyCell division cycle 25A isoform a antibodyCell division cycle 25A isoform b antibodyD9Ertd393e antibodyDual specificity phosphatase CDC25A antibodyEC 3.1.3.48 antibodyM phase inducer phosphatase 1 antibodyM-phase inducer phosphatase 1 antibodyMGC115549 antibodyMPIP1_HUMAN antibody
        see all

      Anti-Cdc25A antibody images

      • Western blot - Cdc25A antibody (ab92892)
        Western blot - Cdc25A antibody (ab92892)
        All lanes : Anti-Cdc25A antibody (ab92892) at 1/1000 dilution

        Lane 1 : HeLa cell lysate at 3 µg
        Lane 2 : HeLa cell lysate at 10 µg
        Lane 3 : HeLa cell lysate at 30 µg


        Predicted band size : 59 kDa

      References for Anti-Cdc25A antibody (ab92892)

      ab92892 has not yet been referenced specifically in any publications.

      Publishing research using ab92892 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab92892 Submit a Question for ab92892

      Displaying 1 - 1 of 1 results for Abreviews and Q&A

      10
      Votes: Highest First

      We purchased this antibody from your company. Should we aliquot this antibody when store it at -20 degree centigrade? As it contains glycerol, repeated freeze thaw cycles can be avoid even not aliquoted.

      Thank you for contacting us. despite being provided in a 50% glycerol solution we would still recommend aliquoting this antibody prior to storing at -20°C. This avoids repeated freeze/thaw cycles and the detrimental effect this can have on the ant...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"