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Product Name
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Cdc25A antibody
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See all Cdc25A antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdc25A
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Immunogen
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Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 1 - 4 µg/ml. WB: 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear membrane
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Research areas
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Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Relevance
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Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdc25A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cdc25A antibody images, references, products related to ab989 and other tools.
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Cdc25A antibody images:
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 Western blot
Lane 1 : Cdc25A antibody (ab989) at 1/2000 dilution
Lane 2 : Cdc25A antibody (ab991) at 1/2000 dilution
Lane 1 : 500ug HeLa whole cell extract, immuncoprecipitated then western blotted.
Lane 2 : 500ug HeLa whole cell extract, immuncoprecipitated then western blotted.
developed using the ECL technique
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Cdc25A
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Cdc25A antibody - more information
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Customer reviews (feedback) regarding Cdc25A antibody |
Customer FAQs regarding Cdc25A antibody |
Protocols for Cdc25A antibody |
Price and availability of products related to Cdc25A antibody |
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Cdc25A antibody - related products:
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Cdc25A antibody - other tools:
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Contact Abcam with a Technical Enquiry about Cdc25A antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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