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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Overview
Product name
Anti-Cdc25A antibody
See all Cdc25A products (18) ...
Description
Rabbit polyclonal to Cdc25A
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 1 - 4 µg/ml.
WB: 1/1000 - 1/10000.Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
Target
Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Domain
The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
Post-translational
modifications
Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
Target information above from: UniProt accessionP30304
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
see all
Database links
- Entrez Gene: 993 Human
- Omim: 116947 Human
- SwissProt: P30304 Human
- Unigene: 437705 Human
Anti-Cdc25A antibody images:
Western blot
Lane 1 : Anti-Cdc25A antibody (ab989) at 1/2000 dilution
Lane 2 : Anti-Cdc25A antibody (ab991) at 1/2000 dilution
Lane 1 : 500ug HeLa whole cell extract, immuncoprecipitated then western blotted.
Lane 2 : 500ug HeLa whole cell extract, immuncoprecipitated then western blotted.
developed using the ECL technique
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
References for Anti-Cdc25A antibody (ab989)
This product has been referenced in:
- Liu Het al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB; Human.Read more (PubMed: 20818375) »
See 1 publication for this product
Publishing research using ab989? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"