Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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ab18321 has been referenced in 4 publications.
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Predicted band size : 59 kDa
Hela cells were treated with 300 µM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs. Nuclear extract was prepared, resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibodies reactive against phosphorylated Cdc25A (Ser17). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
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