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Western blot - Cdc25A (phospho S17) antibody (ab18321)
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Product Name
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Cdc25A (phospho S17) antibody
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See all Cdc25A antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdc25A (phospho S17)
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Immunogen
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Synthetic peptide (Human).
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Reacts with
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Hu
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Tested applications
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IHC-P, WB
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Abreviews
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Application notes
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Recommended dilutions
IHC-P: Use at a concentration of 10 µg/ml.
WB: 1/500. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear membrane
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Research areas
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Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Relevance
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Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdc25A (phospho S17) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cdc25A (phospho S17) antibody images, references, products related to ab18321 and other tools.
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Cdc25A (phospho S17) antibody images:
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 Western blot - Cdc25A (phospho S17) antibody (ab18321)
Predicted band size : 59 kDa
Hela cells were treated with 300 µM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs. Nuclear extract was prepared, resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibodies reactive against phosphorylated Cdc25A (Ser17). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
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References for Cdc25A (phospho S17) antibody (ab18321)
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This product has been used in:
Wang Z et al. Involvement of ATM-mediated Chk1/2 and JNK kinase signaling activation in HKH40A-induced cell growth inhibition. J Cell Physiol 221:213-20 (2009). WB; Human. PubMed: 19530246
If you publish research using ab18321 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cdc25A
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Cdc25A (phospho S17) antibody - more information
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