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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Overview
Product name
Anti-Cdc25A (phospho S75) antibody
See all Cdc25A products (18) ...
Description
Rabbit polyclonal to Cdc25A (phospho S75)
Specificity
Detects endogenous levels of Cdc25A only when phosphorylated at serine 75.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized phosphopeptide derived from Human Cdc25A around the phosphorylation site of serine 75 (M-G-SP-S-E).
Positive control
A2780 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Western blot - Cdc25A (phospho S75) antibody (ab47279)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ELISA
ELISA: 1/10,000ELISA: 1/10,000
Target
Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Domain
The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
Post-translational
modifications
Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
Target information above from: UniProt accessionP30304
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
see all
Database links
- Entrez Gene: 993 Human
- Entrez Gene: 12530 Mouse
- Entrez Gene: 171102 Rat
- Omim: 116947 Human
- SwissProt: P30304 Human
- SwissProt: P48964 Mouse
- SwissProt: Q80VD9 Mouse
- SwissProt: P48965 Rat
see all
Anti-Cdc25A (phospho S75) antibody images:
Western blot - Cdc25A (phospho S75) antibody (ab47279)
All lanes : Anti-Cdc25A (phospho S75) antibody (ab47279) at 1/500 dilution
Lane 1 : Extracts from A2780 cells. UV + no blocking peptide.
Lane 2 : Extracts from A2780 cells. Without UV + without blocking peptide.
Predicted band size : 59 kDa
References for Anti-Cdc25A (phospho S75) antibody (ab47279)
This product has been referenced in:
- Vo NTet al. Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells. Carcinogenesis 31:2074-81 (2010). WB; Human.Read more (PubMed: 19696164) »
See 1 publication for this product
Publishing research using ab47279? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"