Cdc25A (phospho S75) antibody (ab47279)

Product Name 

Cdc25A (phospho S75) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Cdc25A (phospho S75)

Immunogen 

Synthesized phosphopeptide derived from Human Cdc25A around the phosphorylation site of serine 75 (M-G-S^P-S-E).

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

Detects endogenous levels of Cdc25A only when phosphorylated at serine 75.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10,000.
WB: 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A2780 cell extracts.

Cellular localization 

Nuclear membrane

Research areas 

Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases

Relevance 

Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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