![Western blot - Cdc25C antibody [E303] (ab32050) image](#ab3250wb.jpg)
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Product name
Anti-Cdc25C antibody [E303]
See all Cdc25C products (15) ...
Description
Rabbit monoclonal [E303] to Cdc25C
Specificity
ab32050 recognises Cdc25C. It does not cross react with other MPI phosphatase family members.
Tested applications
WB, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in the C terminus of human Cdc25C.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E303
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Western blot - Cdc25C antibody [E303] (ab32050)
![Western blot - Cdc25C antibody [E303] (ab32050) image](/ps/datasheet/Images/32/ab32050/ab3250wb.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
Flow Cyt
Flow Cyt: 1/10.Flow Cyt: 1/10.
IP
IP: 1/30.IP: 1/30.
Application notes
Is unsuitable for ,ICC/IF or IHC.
Target
Function
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Developmental stage
Expressed predominantly in G2 phase.
Post-translational
modifications
Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP30307
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDC 25 antibody
- Cdc 25C antibody
- CDC25 antibody
see all
Database links
- Entrez Gene: 995 Human
- Omim: 157680 Human
- SwissProt: P30307 Human
- Unigene: 656 Human
Anti-Cdc25C antibody [E303] images:
Western blot - Cdc25C antibody [E303] (ab32050)
Anti-Cdc25C antibody [E303] (ab32050) at 1/500 dilution + A431 cell lysate
Predicted band size : 53 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
References for Anti-Cdc25C antibody [E303] (ab32050)
This product has been referenced in:
- Subramaniam D et al. Translation inhibition during cell cycle arrest and apoptosis: Mcl-1 is a novel target for RNA binding protein CUGBP2. Am J Physiol Gastrointest Liver Physiol 294:G1025-32 (2008).Read more (PubMed: 18292181) »
See 1 publication for this product
Publishing research using ab32050? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"