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Anti-Cdc25C (phospho S216) antibody [E190] (ab32051)

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Overview

Product name

Anti-Cdc25C (phospho S216) antibody [E190]
See all Cdc25C products (15) ...

Description

Rabbit monoclonal [E190] to Cdc25C (phospho S216)

Specificity

The antibody only detects Cdc25C phosphorylated on Serine 216.

Tested applications

WB, ICC, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Ser216 of human Cdc25C

Positive control

HeLa cell lysates (intact untreated HeLa cells lysates and HeLa cells lysates treated with Alkaline Phosphatase ( blocking phosphorylation)).

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

E190

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Phospatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases

  • Western blot - Cdc25C (phospho S216) antibody [E190] (ab32051)Western blot - Cdc25C (phospho S216) antibody [E190] (ab32051) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/50.
IP: 1/30.
WB: 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).

Is unsuitable for FACS or IHC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.

Sequence similarities

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Developmental stage

Expressed predominantly in G2 phase.

Post-translational
modifications

Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP30307 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CDC 25 antibody
  • Cdc 25C antibody
  • CDC25 antibody
  • CDC25C antibody
  • Cell division cycle 25 homolog C antibody
  • Cell Division Cycle 25C antibody
  • Cell Division Cycle 25C antibody
  • Cell division cycle 25C protein antibody
  • Dual specificity phosphatase Cdc25C antibody
  • M phase inducer phosphatase 3 antibody
  • M-phase inducer phosphatase 3 antibody
  • Mitosis inducer CDC25 antibody
  • MPIP3 antibody
  • MPIP3_HUMAN antibody
  • Phosphotyrosine phosphatase antibody
see all

Database links

Anti-Cdc25C (phospho S216) antibody [E190] images:

  Western blot - Cdc25C (phospho S216) antibody [E190] (ab32051)

Western blot - Cdc25C (phospho S216) antibody [E190] (ab32051)

Lane 1 : Anti-Cdc25C (phospho S216) antibody [E190] (ab32051) at 1/1000 dilution (Untreated )
Lane 2 : Anti-Cdc25C (phospho S216) antibody [E190] (ab32051) at 1/1000 dilution (Treated with phosphatase)

Lane 1 : As above
Lane 2 : As above


Predicted band size : 53 kDa
Observed band size : 53,60 kDa (why is the actual band size different from the predicted?)

References for Anti-Cdc25C (phospho S216) antibody [E190] (ab32051)

This product has been referenced in:

  • Subramaniam D  et al. Translation inhibition during cell cycle arrest and apoptosis: Mcl-1 is a novel target for RNA binding protein CUGBP2. Am J Physiol Gastrointest Liver Physiol 294:G1025-32 (2008).Read more (PubMed: 18292181) »

See 1 publication for this product

Publishing research using ab32051? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"