If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab72217? Please let us know so that we can cite the reference in this datasheet
ab72217 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Cdc27 antibody (ab72217) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 92 kDa
Observed band size : 92 kDa
Additional bands at : 55 kDa,60 kDa,68 kDa. We are unsure as to the identity of these extra bands.
Detection of Human Cdc27 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72217 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
0
Call 01223 696 000 or contact us