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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-Cdc27 antibody
See all Cdc27 products (7) ...
Description
Rabbit polyclonal to Cdc27
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 774 and 824 of human Cdc27 (NP_001247.2).
Positive control
Whole cell lysates from HeLa and 293T cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats.
Post-translational
modifications
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP30260
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
Database links
- Entrez Gene: 996 Human
- Omim: 116946 Human
- SwissProt: P30260 Human
- Unigene: 463295 Human
Anti-Cdc27 antibody images:
Western blot - Cdc27 antibody (ab72217)
All lanes : Anti-Cdc27 antibody (ab72217) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 92 kDa
Observed band size : 92 kDa
Additional bands at : 55 kDa,60 kDa,68 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Cdc27 antibody (ab72217)
Detection of Human Cdc27 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72217 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
References for Anti-Cdc27 antibody (ab72217)
ab72217 has not yet been referenced specifically in any publications.
Publishing research using ab72217? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"