Recombinant Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdc37 (phospho S13) antibody [EPR4879]
See all Cdc37 primary antibodies -
Description
Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13) -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell, Mouse brain tissue, and Rat brain tissue lysates. Dot: Cdc37 (pS13) phospho peptide.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4879 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
|
Dot blot |
1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
Dot blot
1/1000. |
Target
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Function
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
Sequence similarities
Belongs to the CDC37 family. -
Post-translational
modificationsConstitutively sumoylated by UBE2I. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11140 Human
- Entrez Gene: 12539 Mouse
- Entrez Gene: 114562 Rat
- Omim: 605065 Human
- SwissProt: Q16543 Human
- SwissProt: Q61081 Mouse
- SwissProt: Q63692 Rat
- Unigene: 160958 Human
see all -
Alternative names
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
Images
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All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours, then the membrane treated with Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Dot blot analysis of Cdc37 (pS13) phospho peptide (lane 1) and Cdc37 non-phospho peptide (lane 2) labelling Cdc37 (phospho S13) with ab108360 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/10000 dilution (Purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse brain lysate, then the membrane treated with Phosphatase
Lane 3 : Rat brain lysate
Lane 4 : Rat brain lysate, then the membrane treated with Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab108360 has been referenced in 6 publications.
- D'Amore C et al. Deciphering the role of protein kinase CK2 in the maturation/stability of F508del-CFTR. Biochim Biophys Acta Mol Basis Dis 1866:165611 (2020). PubMed: 31740403
- Borgo C et al. A N-terminally deleted form of the CK2a' catalytic subunit is sufficient to support cell viability. Biochem Biophys Res Commun 531:409-415 (2020). PubMed: 32800562
- Lettieri A et al. Protein Kinase CK2 Subunits Differentially Perturb the Adhesion and Migration of GN11 Cells: A Model of Immature Migrating Neurons. Int J Mol Sci 20:N/A (2019). PubMed: 31779225
- Dushukyan N et al. Phosphorylation and Ubiquitination Regulate Protein Phosphatase 5 Activity and Its Prosurvival Role in Kidney Cancer. Cell Rep 21:1883-1895 (2017). WB ; Human . PubMed: 29141220
- Oberoi J et al. Structural and functional basis of protein phosphatase 5 substrate specificity. Proc Natl Acad Sci U S A 113:9009-14 (2016). WB ; Human . PubMed: 27466404
- Herhaus L et al. Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Sci Signal 8:ra35 (2015). PubMed: 25872870