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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-Cdc37 (phospho S13) antibody
See all Cdc37 products (9) ...
Description
Rabbit polyclonal to Cdc37 (phospho S13)
Specificity
ab61797 detects Cdc37 only when phosphorylated at serine 13.
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human Cdc37 around the phosphorylation site of serine 13 (E-V-SP-D-D).
Positive control
Human placenta tissue and NIH 3T3 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61797 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdk Regulators
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Target
Function
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
Sequence similarities
Belongs to the CDC37 family.
Post-translational
modifications
Constitutively sumoylated by UBE2I.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ16543
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- S cerevisiae hypothetical protein CDC37 antibody
- CDC 37 antibody
- CDC37 antibody
see all
Database links
- Entrez Gene: 11140 Human
- Entrez Gene: 12539 Mouse
- Omim: 605065 Human
- SwissProt: Q16543 Human
- SwissProt: Q61081 Mouse
- Unigene: 160958 Human
- Unigene: 32331 Mouse
Anti-Cdc37 (phospho S13) antibody images:
Western blot - Cdc37 (phospho S13) antibody (ab61797)
All lanes : Anti-Cdc37 (phospho S13) antibody (ab61797) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts
Lane 2 : NIH 3T3 cell extracts with immunising peptide
Predicted band size : 44 kDa
Observed band size : 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdc37 (phospho S13) antibody (ab61797)
ab61797 at 1/50 - 1/100 dilution staining Cdc37 in human placenta by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
References for Anti-Cdc37 (phospho S13) antibody (ab61797)
This product has been referenced in:
- Wang Xet al. The regulatory mechanism of Hsp90alpha secretion and its function in tumor malignancy. Proc Natl Acad Sci U S A 106:21288-93 (2009).Read more (PubMed: 19965370) »
See 1 publication for this product
Publishing research using ab61797? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"