Recombinant Anti-CDC7 antibody [EPR4730] (ab108332)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4730] to CDC7
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CDC7 antibody [EPR4730]
See all CDC7 primary antibodies -
Description
Rabbit monoclonal [EPR4730] to CDC7 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa, and PC3 cell lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4730 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
Target
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Function
Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8317 Human
- Entrez Gene: 12545 Mouse
- Entrez Gene: 360908 Rat
- Omim: 603311 Human
- SwissProt: O00311 Human
- SwissProt: Q9Z0H0 Mouse
- Unigene: 533573 Human
- Unigene: 20842 Mouse
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Alternative names
- Cdc 7 antibody
- CDC7 antibody
- CDC7 cell division cycle 7 antibody
see all
Images
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All lanes : Anti-CDC7 antibody [EPR4730] (ab108332) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa -
ab108332, at 1/100 dilution, staining CDC7 in permeabilized Jurkat cells by intracellular flow cytometry (red). Rabbit IgG (green).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab108332 has been referenced in 3 publications.
- Li Q et al. CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma. Transl Oncol 11:300-306 (2018). PubMed: 29413763
- Pérez-Olivares M et al. Functional interplay between c-Myc and Max in B lymphocyte differentiation. EMBO Rep 19:N/A (2018). PubMed: 30126925
- Zhen H et al. Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells. Evid Based Complement Alternat Med 2014:121276 (2014). WB ; Human . PubMed: 24511319