Overview

  • Product nameAnti-Cdc7 Kinase antibodySee all Cdc7 Kinase primary antibodies ...
  • Description
    Rabbit polyclonal to Cdc7 Kinase
  • Specificityab69950 detects endogenous levels of total Cdc7 Kinase protein.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Cdc7 Kinase

  • Positive control
    • Jurkat cell extracts.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab69950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab69950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ELISA 1/5000.

Target

  • FunctionSeems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc 7 antibody
    • Cdc7 antibody
    • CDC7 cell division cycle 7 antibody
    • CDC7 cell division cycle 7 like 1 antibody
    • CDC7 L1 antibody
    • Cdc7 like 1 antibody
    • CDC7 related kinase antibody
    • CDC7-related kinase antibody
    • CDC7_HUMAN antibody
    • CDC7L 1 antibody
    • CDC7L1 antibody
    • Cell division cycle 7 homolog antibody
    • Cell division cycle 7 kinase antibody
    • Cell division cycle 7 like protein 1 antibody
    • Cell division cycle 7 related protein kinase antibody
    • Cell division cycle 7-related protein kinase antibody
    • HsCDC 7 antibody
    • HsCDC7 antibody
    • Hsk 1 antibody
    • Hsk1 antibody
    • HuCDC 7 antibody
    • huCdc7 antibody
    • MGC117361 antibody
    • MGC126237 antibody
    • MGC126238 antibody
    see all

Anti-Cdc7 Kinase antibody images

  • All lanes : Anti-Cdc7 Kinase antibody (ab69950) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa

References for Anti-Cdc7 Kinase antibody (ab69950)

ab69950 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 50 µg
Specification HEK293T
Treatment Transfected with GFP-Cdc7
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Mar 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"