Anti-Cdk2 antibody [2B6] - BSA and Azide free (ab77671)
Overview
- Product nameAnti-Cdk2 antibody [2B6] - BSA and Azide freeSee all Cdk2 primary antibodies ...
- DescriptionMouse monoclonal [2B6] to Cdk2 - BSA and Azide free
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Recombinant full length protein (Human)
- Positive controlHeLa cells, Tonsil and Rat spleen tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 10mM PBS, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone number2B6
- IsotypeIgG2b
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab77671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IHC-P | |
| ICC/IF |
IHC-P: 1/25 - 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
- Unigene: 689624 Human
- Unigene: 111326 Mouse
- Unigene: 104460 Rat
see all
Target information above from: UniProt accession
P24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cdc2 related protein kinase antibodycdc2-related protein kinase antibodyCdk 2 antibody
- CDK2 antibodyCDK2_HUMAN antibodyCell devision kinase 2 antibodyCell division kinase 2 antibodyCell division protein kinase 2 antibodyCyclin dependent kinase 2 antibodycyclin dependent kinase 2-alpha antibodyCyclin-dependent kinase 2 antibodyp33 protein kinase antibodyp33(CDK2) antibody
see all
Anti-Cdk2 antibody [2B6] - BSA and Azide free images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 antibody [2B6] - BSA and Azide free (ab77671)](http://a.abcam.com/ps/datasheet/Images/77/ab77671/ab77671.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 antibody [2B6] - BSA and Azide free (ab77671)Formalin-fixed, paraffin-embedded rat spleen stained with ab77671, at 1/25 dilution, using a peroxidase-conjugate secondary and AEC chromogen. Note nuclear staining of lymphocytes. This image was generated with a form of this antibody which contains sodium azide and BSA. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 antibody [2B6] - BSA and Azide free (ab77671)](http://a.abcam.com/ps/datasheet/Images/77/ab77671/ab77671 IHC.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 antibody [2B6] - BSA and Azide free (ab77671)Formalin-fixed, paraffin-embedded human tonsil stained with ab77671, at 1/25 dilution, using a peroxidase-conjugate secondary and AEC chromogen. Note nuclear staining of lymphocytes. This image was generated with a form of this antibody which contains sodium azide and BSA.
References for Anti-Cdk2 antibody [2B6] - BSA and Azide free (ab77671)
ab77671 has not yet been referenced specifically in any publications.
