|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cdk2 antibody [2B6](ab2363)
](\ps\datasheet\images\2\ab2363\Cdk2-Primary-antibodies-ab2363-3.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Cdk2 antibody [2B6]
|
| |
See all Cdk2 antibodies (16)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [2B6] to Cdk2
|
|
Immunogen
|
Recombinant full length protein (Human).
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
This antibody reacts with a 33 kD protein known as cdk2.
|
|
Tested applications
(see key)
|
IHC-Fr, IHC-P, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 30 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining.
WB: 1/100 - 1/500. Predicted molecular weight: 34 kDa.
IP: 1/100 - 1/500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Tonsil
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
|
|
Relevance
|
Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
2B6
|
|
Isotype
|
IgG2b
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.100 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk2 antibody [2B6] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Cdk2 antibody [2B6] images, references, products related to ab2363 and other tools.
|
| |
Cdk2 antibody [2B6] images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cdk2 antibody [2B6](ab2363)
Ab2363 staining human placenta. Staining is localized to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
|
| |
References for Cdk2 antibody [2B6] (ab2363)
|
|
This product has been used in:
Gibbs JD et al. Terminal myeloid differentiation is uncoupled from cell cycle arrest. Cell Cycle 6:1205-9 (2007). PubMed: 17495532
If you publish research using ab2363 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Cdk2
|
| |
Cdk2 antibody [2B6] - more information
|
Customer reviews (feedback) regarding Cdk2 antibody [2B6] |
Customer FAQs regarding Cdk2 antibody [2B6] |
Protocols for Cdk2 antibody [2B6] |
Price and availability of products related to Cdk2 antibody [2B6] |
| |
Cdk2 antibody [2B6] - related products:
|
|
|
| |
Cdk2 antibody [2B6] - other tools:
|
Contact Abcam with a Technical Enquiry about Cdk2 antibody [2B6] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
](/index.html?datasheet=2363#Cdk2-Primary-antibodies-ab2363-3.jpg)
