Overview

  • Product nameAnti-Cdk2 antibody [2B6]See all Cdk2 primary antibodies ...
  • Description
    Mouse monoclonal [2B6] to Cdk2
  • SpecificityThis antibody reacts with a 33 kD protein known as cdk2.
  • Tested applicationsFlow Cyt, IHC-P, IHC-Fr, WB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab2363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use 1µg for 106 cells.
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/25 - 1/50. ABC method.
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
IP 1/100 - 1/500.

Target

Anti-Cdk2 antibody [2B6] images

  • Ab2363 staining human placenta. Staining is localized to the nucleus.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing HeLa cells stained with ab2363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Cdk2 antibody [2B6] (ab2363)

This product has been referenced in:
  • Ramathal C  et al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol 30:1607-19 (2010). IHC-P ; Mouse . Read more (PubMed: 20086097) »
  • Medendorp K  et al. The renal cell carcinoma-associated oncogenic fusion protein PRCCTFE3 provokes p21 WAF1/CIP1-mediated cell cycle delay. Exp Cell Res 315:2399-409 (2009). Read more (PubMed: 19422821) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

For ab6538 and ab128167, we source these antibodies from an outside lab so they consider the immunogen sequences to be proprietary. If you can send me the a.a. sequence of the drosophila CDK2 I can ask them to BLAST ...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Oct 25 2011

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MCF-7)
Total protein in input 5 µg
Specification MCF-7
Treatment Commercial CDK2 inhibtor, Roscovitine
Immuno-precipitation step Protein G
Username

Dr. Dean Heathcote

Verified customer

Submitted Jun 16 2009

Application Western blot
Sample Human Cell lysate - whole cell (MCF-7)
Loading amount 5 µg
Specification MCF-7
Treatment CDK7 inhibitors
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Dean Heathcote

Verified customer

Submitted Jun 12 2009

It is approx 100 ug/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"