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Anti-Cdk2 antibody [2B6] (ab2363)

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Overview

Product name

Anti-Cdk2 antibody [2B6]
See all Cdk2 products (19) ...

Description

Mouse monoclonal [2B6] to Cdk2

Specificity

This antibody reacts with a 33 kD protein known as cdk2.

Tested applications

IHC-P, IHC-Fr, WB, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Recombinant full length protein (Human).

Positive control

Tonsil

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

2B6

Myeloma

unknown

Isotype

IgG2b

Light chain type

unknown

Research areas

Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cdk2 antibody [2B6](ab2363)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cdk2 antibody [2B6](ab2363) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.

Target information above from: UniProt accessionP24941 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdc2 related protein kinase antibody
  • Cdk 2 antibody
  • CDK2 antibody
  • CDK2_HUMAN antibody
  • Cell division kinase 2 antibody
  • Cell division protein kinase 2 antibody
  • Cyclin dependent kinase 2 antibody
  • Cyclin-dependent kinase 2 antibody
  • p33 protein kinase antibody
  • p33(CDK2) antibody
see all

Database links

see all

Anti-Cdk2 antibody [2B6] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cdk2 antibody [2B6](ab2363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cdk2 antibody [2B6](ab2363)

Ab2363 staining human placenta. Staining is localized to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Cdk2 antibody [2B6] (ab2363)

This product has been referenced in:

  • Ramathal Cet al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol 30:1607-19 (2010). IHC-P; Mouse.Read more (PubMed: 20086097) »
  • Medendorp Ket al. The renal cell carcinoma-associated oncogenic fusion protein PRCCTFE3 provokes p21 WAF1/CIP1-mediated cell cycle delay. Exp Cell Res 315:2399-409 (2009).Read more (PubMed: 19422821) »

See all 4 publications for this product

Publishing research using ab2363? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"