![Western blot - Cdk2 antibody [E304] (ab32147) image](#ab32147wb.jpg)
![Immunofluorescence - Cdk2 antibody [E304] (ab32147) image](#ab32147IF.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Cdk2 antibody [E304]
See all Cdk2 products (20) ...
Description
Rabbit monoclonal [E304] to Cdk2
Tested applications
ICC/IF, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in C-terminus of human Cdk2
Epitope
The epitope is within the C-terminus of human Cdk2
Positive control
Hela cells Hela cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
E304
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
IP
Application notes
ICC/IF: 1/100.
IP: 1/40.
WB: 1/2000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionP24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- Cdk 2 antibody
see all
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
see all
Anti-Cdk2 antibody [E304] images:
Western blot - Cdk2 antibody [E304] (ab32147)
![Western blot - Cdk2 antibody [E304] (ab32147)](/ps/datasheet/Images/32/ab32147/ab32147wb.jpg)
Anti-Cdk2 antibody [E304] (ab32147) at 1/5000 dilution + Hela cell lysate
Predicted band size : 34 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Immunofluorescence - Cdk2 antibody [E304] (ab32147)
![Immunofluorescence - Cdk2 antibody [E304] (ab32147)](/ps/datasheet/Images/32/ab32147/ab32147IF.jpg)
Immunofluorescent staining of HeLa cells using ab32147
References for Anti-Cdk2 antibody [E304] (ab32147)
ab32147 has not yet been referenced specifically in any publications.
Publishing research using ab32147? Please let us know so that we can cite the reference in this datasheet
![Cdk2 antibody [E304] for WB in Human (32147)](/ps/datasheet/images/32/ab32147/Cdk2-Primary-antibodies-ab32147-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"