Overview

  • Product nameAnti-Cdk2 antibody [E304]
    See all Cdk2 primary antibodies
  • Description
    Rabbit monoclonal [E304] to Cdk2
  • Tested applicationsICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in C-terminus of human Cdk2

  • EpitopeThe epitope is within the C-terminus of human Cdk2
  • Positive control
    • Hela cells Hela cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IP
WB
  • Application notesICC/IF: 1/100.

    IP: 1/40.
    WB: 1/2000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).

    Is unsuitable for FACS.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    Anti-Cdk2 antibody [E304] images

    • All lanes : Anti-Cdk2 antibody [E304] (ab32147) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 34 kDa
      Observed band size : 34 kDa


      Exposure time : 4 minutes

      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab32147 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Immunofluorescent staining of HeLa cells using ab32147

    References for Anti-Cdk2 antibody [E304] (ab32147)

    This product has been referenced in:
    • Le TD  et al. Capsaicin-induced apoptosis of FaDu human pharyngeal squamous carcinoma cells. Yonsei Med J 53:834-41 (2012). WB . Read more (PubMed: 22665354) »
    • Liu D  et al. Lewis y Regulate Cell Cycle Related Factors in Ovarian Carcinoma Cell RMG-I in Vitro via ERK and Akt Signaling Pathways. Int J Mol Sci 13:828-39 (2012). WB . Read more (PubMed: 22312289) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Human Cell (MCF-7)
    Specification MCF-7
    Permeabilization Yes - Triton X-100
    Fixative Paraformaldehyde
    Username

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    Verified customer

    Submitted Aug 04 2014

    Application Western blot
    Loading amount 50000 cells
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Human Cell lysate - whole cell (HEK293, MCF-7, MDA-MB-231)
    Specification HEK293, MCF-7, MDA-MB-231
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Verified customer

    Submitted Aug 04 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (T-47D)
    Loading amount 60 µg
    Specification T-47D
    Treatment 0.08% DMSO for 48 hrs
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-20% Tris-glycine)
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

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    Verified customer

    Submitted Aug 31 2009

    Thank you for your patience. Regarding ab32147 (Cdk2 antibody), the exact immunogen sequence and amino acid location is proprietary information. At this time, we have not tested ab32147 for cross-reactivity with Cdk1. Please don't hesitate to co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"