Overview
- Product nameAnti-Cdk2 antibodySee all Cdk2 primary antibodies ...
- DescriptionRabbit polyclonal to Cdk2
- SpecificityDetects endogenous levels of total Cdk2 protein.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat - Immunogen
Synthetic peptide from around the phosphorylation site of Threonine 160 of Human Cdk2
- Positive controlA2780 cells. MDA MB 435 cells.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab47518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
| ELISA | ELISA: 1/10000. |
Target
- FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
- Unigene: 689624 Human
- Unigene: 111326 Mouse
- Unigene: 104460 Rat
see all
Target information above from: UniProt accession
P24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cdc2 related protein kinase antibodycdc2-related protein kinase antibodyCdk 2 antibody
- CDK2 antibodyCDK2_HUMAN antibodyCell devision kinase 2 antibodyCell division kinase 2 antibodyCell division protein kinase 2 antibodyCyclin dependent kinase 2 antibodycyclin dependent kinase 2-alpha antibodyCyclin-dependent kinase 2 antibodyp33 protein kinase antibodyp33(CDK2) antibody
see all
Anti-Cdk2 antibody images
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Western blot - Cdk2 antibody (ab47518)All lanes : Anti-Cdk2 antibody (ab47518)
Lane 1 : A2780 cell extract
Lane 2 : MDA MB 435 cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size : 34 kDa
Observed band size : 34 kDa
References for Anti-Cdk2 antibody (ab47518)
This product has been referenced in:
- Tomashevski A et al. Cyclin-C-dependent cell-cycle entry is required for activation of non-homologous end joining DNA repair in postmitotic neurons. Cell Death Differ 17:1189-98 (2010). WB ; Rat . Read more (PubMed: 20111042) »
