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Anti-Cdk2 antibody (ab47518)

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Overview

Product name

Anti-Cdk2 antibody
See all Cdk2 products (19) ...

Description

Rabbit polyclonal to Cdk2

Specificity

Detects endogenous levels of total Cdk2 protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide from around the phosphorylation site of Threonine 160 of Human Cdk2

Positive control

A2780 cells. MDA MB 435 cells.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

  • Western blot - Cdk2 antibody (ab47518)Western blot - Cdk2 antibody (ab47518) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab47518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

Target

Function

Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.

Target information above from: UniProt accessionP24941 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdc2 related protein kinase antibody
  • Cdk 2 antibody
  • CDK2 antibody
  • CDK2_HUMAN antibody
  • Cell division kinase 2 antibody
  • Cell division protein kinase 2 antibody
  • Cyclin dependent kinase 2 antibody
  • Cyclin-dependent kinase 2 antibody
  • p33 protein kinase antibody
  • p33(CDK2) antibody
see all

Database links

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Anti-Cdk2 antibody images:

  Western blot - Cdk2 antibody (ab47518)

Western blot - Cdk2 antibody (ab47518)

All lanes : Anti-Cdk2 antibody (ab47518)

Lane 1 : A2780 cell extract
Lane 2 : MDA MB 435 cell extract

Lysates/proteins at 30 µg per lane.


Predicted band size : 34 kDa
Observed band size : 34 kDa

References for Anti-Cdk2 antibody (ab47518)

This product has been referenced in:

  • Tomashevski Aet al. Cyclin-C-dependent cell-cycle entry is required for activation of non-homologous end joining DNA repair in postmitotic neurons. Cell Death Differ 17:1189-98 (2010). WB; Rat.Read more (PubMed: 20111042) »

See 1 publication for this product

Publishing research using ab47518? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"