Lanes 1 and 2: Green signal from target – ab64669 observed at 34 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab64669 was shown to react with CDK2 when CDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab64669 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Cdk2 antibody (ab64669)
All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate (ab29889) Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Thymus (Rat) Tissue Lysate Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml) Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 35 kDa Observed band size : 35 kDa Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Cdk2 antibody (ab64669)
has not yet been referenced specifically in any publications.
Publishing research using ab64669? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"