Lanes 1 and 2: Green signal from target – ab64669 observed at 34 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab64669 was shown to react with CDK2 when CDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab64669 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-Cdk2 antibody (ab64669)
All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889) Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Thymus (Rat) Tissue Lysate Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml) Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa Observed band size: 35 kDa Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.