Overview

  • Product nameAnti-Cdk2 antibody
    See all Cdk2 primary antibodies
  • Description
    Rabbit polyclonal to Cdk2
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Chinese Hamster, Syrian Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Cdk2.

    (Peptide available as ab71917.)

  • Positive control
    • This antibody gave a positive signal in the following Lysates: HeLa Whole Cell, Rat Thymus Tissue, HeLa Whole Cell - Bleomycin Treated (20U/ml), Jurkat Whole Cell, Y79 Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab64669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human Cdk2 peptide (ab71917).

Target

  • FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc2 related protein kinase antibody
    • cdc2-related protein kinase antibody
    • CDC28 antibody
    • CDC2A antibody
    • Cdk 2 antibody
    • CDK1 antibody
    • CDK2 antibody
    • CDK2_HUMAN antibody
    • CDKN2 antibody
    • Cell devision kinase 2 antibody
    • Cell division kinase 2 antibody
    • Cell division protein kinase 2 antibody
    • Cyclin dependent kinase 2 antibody
    • cyclin dependent kinase 2-alpha antibody
    • Cyclin-dependent kinase 2 antibody
    • kinase Cdc2 antibody
    • MPF antibody
    • p33 protein kinase antibody
    • p33(CDK2) antibody
    see all

Anti-Cdk2 antibody images

  • All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Thymus (Rat) Tissue Lysate
    Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab64669 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64669, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.

References for Anti-Cdk2 antibody (ab64669)

ab64669 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab64669.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"