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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab6538? Please let us know so that we can cite the reference in this datasheet
ab6538 has been referenced in 1 publications.
- Liu H et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB; Human. PubMed: 20818375
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry - Cdk2 antibody (ab6538)
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated with ab6538 (1/200). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.
Darin McDonald, Cross Cancer Institute
Immunohistochemistry (Frozen sections) - Cdk2 antibody (ab6538)
ab6538 staining Cdk2 in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20ºC. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4ºC. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary.
This image is courtesy of an anonymous Abreview
Western blot - Cdk2 antibody (ab6538)
Predicted band size : 34 kDa
Observed band size : 34 kDa
All Lanes: Cdk2 antibody (ab6538) at 1/1000 dilution + Mouse brain whole tissue lysate (100 µg)
Secondary Antibody: An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 developed using the ECL technique
Blocking Step: 5% Milk for 1 hour at 25 °C.
Gel Running Conditions: Reduced, denaturing.
This image is courtesy of an anonymous Abreview
Cdk2 antibody for Western blot in Human (6538)
Cdk2 antibody for IHC-Fr in Mouse (6538)
Cdk2 antibody for Immunohistochemistry (Frozen sections) in Rat (6538)
Cdk2 antibody for ICC/IF in Mouse (6538)
Cdk2 antibody for ICC/IF in Rat (6538)
Anti-Cdk2 antibody for Western blot in Human (6538)
Cdk2 antibody for Western blot in Mouse (6538)
Cdk2 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (6538)
Cdk2 antibody for ICC/IF in Human (6538)
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