Anti-Cdk2 antibody (ab6538)

The aa sequence for Drosophila CDK2 is:

MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPS
TAIREISLLKNLKHPNVVQLFDVVISGNNLYMIFEYLNMDLKKLMDKKKD
VFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFG
LARAFNVPMRAYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMI
MRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFKTKFPRWEGTN
MPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPV
DPNAGSASRLTRLV

Let me know what you find!

ANSWER:

Thank you for your reply.

For ab6538, the sequence homology for the C-Terminus region is very limited between human and Drosophila, so we wouldn't recommend using it.
Query: 244 PRWEGTNMPQ--PITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNV-QHVDHVAL 298
P+W + + P + + L+ ML YDPN RISAK AL H +F++V + V H+ L
Sbjct: 241 PKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL 298
For ab128167, there is about 70% sequence homology. It may be that this is the best overlap, but it's still below our recommended cut-off of 85%.
I hope this information helps. Please contact us with any other questions.

Great, thanks. That end of the protein is actually *not* very well conserved. Could you provide the antigen for the other antibodies? They are...

ab6538

ab2363

ab128167

P.S., all Drosophila sequences can be found here: http://www.flybase.org/

Thanks for any additional info you can provide!

ANSWER:

Thank you for your reply.

For ab6538 and ab128167, we source these antibodies from an outside lab so they consider the immunogen sequences to be proprietary. If you can send me the a.a. sequence of the drosophila CDK2 I can ask them to BLAST it.

For ab2363, the immunogen was full-length human protein, but the epitope has not been mapped.

http://www.uniprot.org/uniprot/P24941

I hope this information helps. Please contact me with any other questions.

Do you guys have a positive control for Anti-Cdk2 antibody (ab6538)? I would like to purchase this antibody with a tested positive control. Thanks!

sincerely,

ANSWER:

Thank you for contacting us.

I presume you are looking for positive control used in western blot. Our customers have used this ab successfully with mouse brain tissue and 293 cells lysates. Please check the Abreviews. The Mouse brain lysates can be found with catalogue numbers are; ab7189, ab7190 ab30151 and Human 293 cell lysates with catalogue number ab7902.

Please also click the following link to see the tissues which express CDK2 target, lysates of these can be used as positive control.

http://www.proteinatlas.org/ENSG00000123374/normal

http://www.proteinatlas.org/ENSG00000123374/cancer

http://www.proteinatlas.org/ENSG00000123374/cell

We have the tissue lysates in catalogue. Please type "lysates" in the product search box provide on the Abcam homepage and system will give you sorted products as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Do you think that your antibody could cross-react with Xenopus-Cdk2?

ANSWER:

This antibody has not been tested against Xenopus Cdk2. However, the antibody was raised against a peptide from the c-terminal of human Cdk2, which is homologous to the Xenopus sequence. Therefore there is a good possibility that the antibody will crosss-react with Xenopus Cdk2.