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Immunocytochemistry - Cdk2 antibody (ab6538)
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Product Name
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Cdk2 antibody
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See all Cdk2 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdk2
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Immunogen
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Synthetic peptide (Human) conjugated to KLH.
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Reacts with
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Hu, Ms, Rat
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Specificity
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No reaction was observed against other related cyclin dependent kinases.
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Tested applications
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ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ELISA: 1/500 - 1/2000.
ICC/IF: 1/200. Fix cells in 4% paraformaldehyde.Permeabilize in 0.5% Triton X-100.
IHC-P: 1/200 - 1/1000.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Relevance
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Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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This product was prepared from monospecific antiserum by delipidation and defibrination.
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Form
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Liquid
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Concentration
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80.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cdk2 antibody images, references, products related to ab6538 and other tools.
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Cdk2 antibody images:
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 Immunocytochemistry - Cdk2 antibody (ab6538)
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated with ab6538 (1/200). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification. Darin McDonald, Cross Cancer Institute
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Search PubMed (MEDLINE) for references to Cdk2
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Cdk2 antibody - more information
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Customer reviews (feedback) regarding Cdk2 antibody |
Customer FAQs regarding Cdk2 antibody |
Protocols for Cdk2 antibody |
Price and availability of products related to Cdk2 antibody |
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Cdk2 antibody - related products:
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Cdk2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Cdk2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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