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Immunocytochemistry - Cdk2 antibody (ab6538)
Immunocytochemistry - Cdk2 antibody (ab6538)
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Product Name 

Cdk2 antibody

 

See all Cdk2 antibodies (16)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Cdk2

Immunogen 

Synthetic peptide (Human) conjugated to KLH.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

No reaction was observed against other related cyclin dependent kinases.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


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• ICC/IF

2 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• IHC-Fr

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


ICC/IF
(read Abreview)


ICC/IF
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IHC-Fr
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ELISA: 1/500 - 1/2000.
ICC/IF: 1/200. Fix cells in 4% paraformaldehyde.Permeabilize in 0.5% Triton X-100.
IHC-P: 1/200 - 1/1000.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 34 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

Relevance 

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

 

Alternative names Cdk2 antibody (ab6538)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1017    (Human)
12566    (Mouse)
362817    (Rat)

Omim

    

116953    (Human)

SwissProt

    

P24941    (Human)
P97377    (Mouse)
Q63699    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

This product was prepared from monospecific antiserum by delipidation and defibrination.

Form 

Liquid

Concentration 

80.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Cdk2 antibody images, references, products related to ab6538 and other tools.

 

Cdk2 antibody images:

Product image  Immunocytochemistry - Cdk2 antibody (ab6538)

Immunocytochemistry - Cdk2 antibody (ab6538)

SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated with ab6538 (1/200). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.

Darin McDonald, Cross Cancer Institute

 
 

Search PubMed (MEDLINE) for references to Cdk2

 

Cdk2 antibody - more information

Customer reviews (feedback) regarding Cdk2 antibody

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Protocols for Cdk2 antibody

Price and availability of products related to Cdk2 antibody

 

Cdk2 antibody - related products:

Products for Immunocytochemistry

Subcellular Markers

Products for Immunofluorescence

Subcellular Markers

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

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