Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Overview
Product name
Anti-Cdk2 antibody
See all Cdk2 products (19) ...
Description
Rabbit polyclonal to Cdk2
Specificity
No reaction was observed against other related cyclin dependent kinases.
Tested applications
ICC/IF, ELISA, IHC-P, IP, WB, IHC-Fr, ICC, IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) conjugated to KLH.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None, 0.05% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate, pH 7.6
Purity
Whole antiserum
Purification notes
This product was prepared from monospecific antiserum by delipidation and defibrination.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (Fix cells in 4% paraformaldehyde. Permeabilize in 0.5% Triton X-100.)
ELISA
ELISA: 1/500 - 1/2000.ELISA: 1/500 - 1/2000.
IHC-P: 1/200 - 1/1000.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000.Predicted molecular weight: 34 kDa.
IHC-Fr: 1/250
ICC: 1/200
IF
IF: 1/200IF: 1/200
Target
Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionP24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc2 related protein kinase antibody
- Cdk 2 antibody
- CDK2 antibody
see all
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
see all
Anti-Cdk2 antibody images:
Immunocytochemistry - Cdk2 antibody (ab6538)
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated with ab6538 (1/200). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.
Darin McDonald, Cross Cancer Institute
Immunohistochemistry (Frozen sections) - Cdk2 antibody (ab6538)
ab6538 staining Cdk2 in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary.
This image is courtesy of an anonymous Abreview
Western blot - Cdk2 antibody (ab6538)
Predicted band size : 34 kDa
Observed band size : 34 kDa
All Lanes: Cdk2 antibody (ab6538) at 1/1000 dilution + Mouse brain whole tissue lysate (100 µg)
Secondary Antibody: An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 developed using the ECL technique
Blocking Step: 5% Milk for 1 hour at 25 °C.
Gel Running Conditions: Reduced, denaturing.
This image is courtesy of an anonymous Abreview
References for Anti-Cdk2 antibody (ab6538)
This product has been referenced in:
- Liu Het al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB; Human.Read more (PubMed: 20818375) »
See 1 publication for this product
Publishing research using ab6538? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"