Overview
- Product nameAnti-Cdk2 antibodySee all Cdk2 primary antibodies ...
- DescriptionRabbit polyclonal to Cdk2
- SpecificityNo reaction was observed against other related cyclin dependent kinases.
- Tested applicationsICC/IF, ELISA, IHC-P, IP, WB, IHC-Fr, ICC more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide (Human) conjugated to KLH.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None, 0.05% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate, pH 7.6 - PurityWhole antiserum
- Purification notesThis product was prepared from monospecific antiserum by delipidation and defibrination.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. Fix cells in 4% paraformaldehyde. Permeabilize in 0.5% Triton X-100. |
| ELISA | ELISA: 1/500 - 1/2000. |
| IHC-P | IHC-P: 1/200 - 1/1000. |
| IP | IP: Use at an assay dependent dilution. |
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 34 kDa. |
| IHC-Fr | IHC-Fr: 1/250. |
| ICC | ICC: 1/200. |
Target
- FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
- Unigene: 689624 Human
- Unigene: 111326 Mouse
- Unigene: 104460 Rat
see all
Target information above from: UniProt accession
P24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cdc2 related protein kinase antibodycdc2-related protein kinase antibodyCdk 2 antibody
- CDK2 antibodyCDK2_HUMAN antibodyCell devision kinase 2 antibodyCell division kinase 2 antibodyCell division protein kinase 2 antibodyCyclin dependent kinase 2 antibodycyclin dependent kinase 2-alpha antibodyCyclin-dependent kinase 2 antibodyp33 protein kinase antibodyp33(CDK2) antibody
see all
Anti-Cdk2 antibody images
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Immunocytochemistry - Cdk2 antibody (ab6538)Darin McDonald, Cross Cancer InstituteSK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated with ab6538 (1/200). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.
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Immunohistochemistry (Frozen sections) - Cdk2 antibody (ab6538)This image is courtesy of an anonymous Abreviewab6538 staining Cdk2 in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary. -
Western blot - Cdk2 antibody (ab6538)
Predicted band size : 34 kDa
Observed band size : 34 kDaThis image is courtesy of an anonymous Abreview
References for Anti-Cdk2 antibody (ab6538)
This product has been referenced in:
- Gao M et al. A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity. PLoS One 7:e49687 (2012). WB ; Human . Read more (PubMed: 23166748) »
- Liu H et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB ; Human . Read more (PubMed: 20818375) »
