Overview

  • Product nameAnti-Cdk2 antibodySee all Cdk2 primary antibodies ...
  • Description
    Rabbit polyclonal to Cdk2
  • Tested applicationsWB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from an internal region of Human Cdk2.

  • Positive control
    • Extracts from HUVEC, HepG2 or Jurkat cells; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

Anti-Cdk2 antibody images

  • All lanes : Anti-Cdk2 antibody (ab70012) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from Jurkat cells
    Lane 4 : Extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells using 1/500 ab70012. Right: the sample was pre-incubated with the immunizing peptide

References for Anti-Cdk2 antibody (ab70012)

ab70012 has not yet been referenced specifically in any publications.

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