Anti-Cdk2 antibody (ab71919)

Overview

• Product nameAnti-Cdk2 antibodySee all Cdk2 primary antibodies ...
• Description
  Rabbit polyclonal to Cdk2
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Mouse, Rabbit, Dog, Rhesus monkey, Chinese Hamster, Syrian Hamster
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Cdk2.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab71918.)

• Positive controlThis antibody gave a positive signal in the following Lysates: Rat Thymus Tissue, HeLa Whole Cell, TE 671 Whole Cell, HeLa Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Hydroxyurea Treated (48hr, 2uM)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Cdks

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.

Target information above from: UniProt accession P24941 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1017 Human
  • Entrez Gene: 12566 Mouse
  • Entrez Gene: 362817 Rat
  • Omim: 116953 Human
  • SwissProt: P24941 Human
  • SwissProt: P97377 Mouse
  • SwissProt: Q63699 Rat
  • Unigene: 19192 Human

  • Unigene: 689624 Human
  • Unigene: 111326 Mouse
  • Unigene: 104460 Rat

  see all

• Alternative names
  Cdc2 related protein kinase antibodycdc2-related protein kinase antibodyCdk 2 antibody

  CDK2 antibodyCDK2_HUMAN antibodyCell devision kinase 2 antibodyCell division kinase 2 antibodyCell division protein kinase 2 antibodyCyclin dependent kinase 2 antibodycyclin dependent kinase 2-alpha antibodyCyclin-dependent kinase 2 antibodyp33 protein kinase antibodyp33(CDK2) antibody

  see all

Anti-Cdk2 antibody images

• 

  Western blot - Cdk2 antibody (ab71919)
  All lanes : Anti-Cdk2 antibody (ab71919) at 1 µg/ml
  
  Lane 1 : Thymus (Rat) Tissue Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 3 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
  Lane 4 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
  Lane 5 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 35 kDa
  Observed band size : 35 kDa
  Additional bands at : 130 kDa,>250 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - Cdk2 antibody (ab71919)
  ICC/IF image of ab71919 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71919, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.