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Hi-- I am looking for a cdk2 antibody (probably a polyclonal) for whole-mount immunofluorescence in Drosophila ovaries. Based on the immunogen (C-term end of peptide, but not sure exactly what residues?), is this antibody (ab7954) predicted to cross-react with the Drosophila protein? Thanks! |
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Thank you for your inquiry. |
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je voudrais utiliser les anticorps dont les références sont : |
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ANSWER: |
Notre catalogue contient plus de 70 000 références anticorpset nous ne pouvons malheureusement pas offrir, pour des raisons logistiques et de stockage, d'échantillon gratuit ou de petit volume pour des essais préliminaires. |
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I was wondering if your primary antibody to CDK2 (ab7954), was capable of recognizes both the unphosphorylated and phosphorylated forms. Any information you can provide is greatly appreciated. Thank you. |
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ANSWER: |
Thank you for your enquiry. This product will not discriminate between the unphosphorylated and phosporylated forms of Cdk2. So yes it will recognize both forms. If you have any other questions, please do not hesitate to contact me. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 34 kDa
ab7954 staining Cdk2 in HeLa whole cell lysate (lane A), HL-60 whole cell lysate (lane B) and NIH / 3T3 whole cell lysate (lane C).
Synaptonemal complexes (red) from human spermatocytes at the pachytene stage showing CDK2 foci (green) and the kinetochore region (blue).
This picture was kindly supplied as part of the review submitted by Maria Oliver-Bonet.
All lanes : Anti-Cdk2 antibody (ab7954) at 1/200 dilution
Lane 1 : Whole cell lysate prepared from rat primary cortical neurons (untreated)
Lane 2 : Whole cell lysate prepared from rat primary cortical neurons (treated with drug)
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat polyclonal to rabbit Ig at 1/10000 dilution
Performed under non-reducing conditions.
Predicted band size : 34 kDa
Exposure time : 2 minutes
This image is courtesy of an Abreview submitted by Dr Elena Schwartz
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