Overview

  • Product nameAnti-Cdk2 antibody
    See all Cdk2 primary antibodies
  • Description
    Rabbit polyclonal to Cdk2
  • SpecificityReacts with Cdk2 unphosphorylated and phosporylated forms.
  • Tested applicationsIHC-Fr, IP, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive control
    • HeLa, HL-60 and NIH / 3T3 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab7954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
IP Use a concentration of 5 µg/ml.
WB 1/400. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).Can be blocked with Cdk2 peptide (ab8009).
ICC Use at an assay dependent dilution. See Abreview.

Target

  • FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc2 related protein kinase antibody
    • cdc2-related protein kinase antibody
    • CDC28 antibody
    • CDC2A antibody
    • Cdk 2 antibody
    • CDK1 antibody
    • CDK2 antibody
    • CDK2_HUMAN antibody
    • CDKN2 antibody
    • Cell devision kinase 2 antibody
    • Cell division kinase 2 antibody
    • Cell division protein kinase 2 antibody
    • Cyclin dependent kinase 2 antibody
    • cyclin dependent kinase 2-alpha antibody
    • Cyclin-dependent kinase 2 antibody
    • kinase Cdc2 antibody
    • MPF antibody
    • p33 protein kinase antibody
    • p33(CDK2) antibody
    see all

Anti-Cdk2 antibody images



  • Predicted band size : 34 kDa


    ab7954 staining Cdk2 in HeLa whole cell lysate (lane A), HL-60 whole cell lysate (lane B) and NIH / 3T3 whole cell lysate (lane C).
  • Synaptonemal complexes (red) from human spermatocytes at the pachytene stage showing CDK2 foci (green) and the  kinetochore region (blue).

    This picture was kindly supplied as part of the review submitted by Maria Oliver-Bonet.

  • All lanes : Anti-Cdk2 antibody (ab7954) at 1/200 dilution

    Lane 1 : Whole cell lysate prepared from rat primary cortical neurons (untreated)
    Lane 2 : Whole cell lysate prepared from rat primary cortical neurons (treated with drug)

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat polyclonal to rabbit Ig at 1/10000 dilution

    Performed under non-reducing conditions.

    Predicted band size : 34 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Elena Schwartz

    See Abreview

  • Cdk2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cdk2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7954.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 34kDa: Cdk2

References for Anti-Cdk2 antibody (ab7954)

This product has been referenced in:
  • Munkley J  et al. Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Res 39:2671-7 (2011). WB . Read more (PubMed: 21109536) »
  • Hodeify R  et al. Cdk2-dependent phosphorylation of p21 regulates the role of Cdk2 in cisplatin cytotoxicity. Am J Physiol Renal Physiol 300:F1171-9 (2011). IP . Read more (PubMed: 21325496) »

See all 11 Publications for this product

Product Wall

Thank you for contacting us. I ran the Schmidtea Mediterranea sequence against the immunogen sequences for our different Cdk2 antibodies and unfortunately found none that are predicted to react. The most homology was with ab64669, which came to 55%. Ge...

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Thank you for your inquiry.


I could not find the drosophila CDK2 sequence online so I wasn't able to BLAST the immunogen sequence for you. However, if you have the sequence, I can run the BLAST for you. Generally we say that >85% seq...

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Notre catalogue contient plus de 70 000 références anticorpset nous ne pouvons malheureusement pas offrir, pour des raisons logistiques et de stockage, d'échantillon gratuit ou de petit volume pour des essais préliminaires. ...

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Thank you for your enquiry. This product will not discriminate between the unphosphorylated and phosporylated forms of Cdk2. So yes it will recognize both forms. If you have any other questions, please do not hesitate to contact me.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (primary cortical neurons)
Loading amount 20 µg
Specification primary cortical neurons
Gel Running Conditions Non-reduced Denaturing (4-12% Bis-Tris-Gel)
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Elena Schwartz

Verified customer

Submitted Nov 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"