Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

Product Name 

Cdk2 (phospho T14) antibody [EP2234Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP2234Y] to Cdk2 (phospho T14)

Immunogen 

A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.

Reacts with 

Hu, ChHm

Does not react with 
(species key)

Ms, Rat

Specificity 

This antibody detects Cdk2 phosphorylated on Threonine 14.

Tested applications 
(see key)

ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/30.
WB: 1/5000 - 1/20,000. Detects a band of approximately ~34 kDa (predicted molecular weight: 34 kDa).

Is unsuitable for Flow Cyt.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate, human tonsil tissue.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

Relevance 

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP2234Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for Cdk2 (phospho T14) antibody [EP2234Y] images, references, products related to ab68265 and other tools.

Cdk2 (phospho T14) antibody [EP2234Y] images:

References for Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

  This product has been used in:

Prosser SL et al. Molecular dissection of the centrosome overduplication pathway in S-phase-arrested cells. Mol Cell Biol 29:1760-73 (2009). WB; Chinese Hamster. PubMed: 19139275

If you publish research using ab68265 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Cdk2 (phospho T14) antibody [EP2234Y]

Customer FAQs regarding Cdk2 (phospho T14) antibody [EP2234Y]

Protocols for Cdk2 (phospho T14) antibody [EP2234Y]

Price and availability of products related to Cdk2 (phospho T14) antibody [EP2234Y]

Cdk2 (phospho T14) antibody [EP2234Y] - related products: