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Western blot - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)
![Western blot - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)](/ps/datasheet/images/ab68265.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)](/ps/datasheet/images/ab68265ihc.jpg)
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Product Name
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Cdk2 (phospho T14) antibody [EP2234Y]
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See all Cdk2 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP2234Y] to Cdk2 (phospho T14)
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Immunogen
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A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.
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Reacts with
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Hu, ChHm
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Does not react with
(species key)
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Ms, Rat
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Specificity
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This antibody detects Cdk2 phosphorylated on Threonine 14.
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Tested applications
(see key)
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ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC: 1/100 - 1/250. IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IP: 1/30. WB: 1/5000 - 1/20,000. Detects a band of approximately ~34 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for Flow Cyt.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate, human tonsil tissue.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Relevance
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Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP2234Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk2 (phospho T14) antibody [EP2234Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cdk2 (phospho T14) antibody [EP2234Y] images, references, products related to ab68265 and other tools.
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Cdk2 (phospho T14) antibody [EP2234Y] images:
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Western blot - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)
All lanes : Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/20000 dilution
Lane 1 : HeLa cell lysate, untreated. Lane 2 : HeLa cell lysate, treated with hydroxyurea.
Lysates/proteins at 10 µg per lane.
Secondary HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size : 34 kDa Observed band size : ~34 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)
Human tonsil tissue stained with ab68265 at 1/100-1/250 dilution.
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References for Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)
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This product has been used in:
Prosser SL et al. Molecular dissection of the centrosome overduplication pathway in S-phase-arrested cells. Mol Cell Biol 29:1760-73 (2009). WB; Chinese Hamster. PubMed: 19139275
If you publish research using ab68265 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cdk2
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Cdk2 (phospho T14) antibody [EP2234Y] - more information
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Customer reviews (feedback) regarding Cdk2 (phospho T14) antibody [EP2234Y] |
Customer FAQs regarding Cdk2 (phospho T14) antibody [EP2234Y] |
Protocols for Cdk2 (phospho T14) antibody [EP2234Y] |
Price and availability of products related to Cdk2 (phospho T14) antibody [EP2234Y] |
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Cdk2 (phospho T14) antibody [EP2234Y] - related products:
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Cdk2 (phospho T14) antibody [EP2234Y] - other tools:
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Contact Abcam with a Technical Enquiry about Cdk2 (phospho T14) antibody [EP2234Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"