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RabMAbs (2351-1)

Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

Overview

  • Product nameAnti-Cdk2 (phospho T14) antibody [EP2234Y]See all Cdk2 primary antibodies ...
  • Description
    Rabbit monoclonal [EP2234Y] to Cdk2 (phospho T14)
  • SpecificityThis antibody detects Cdk2 phosphorylated on Threonine 14.
  • Tested applicationsWB, IP, IHC-P, ICC more details
  • Species reactivity
    Reacts with: Human, Chinese Hamster

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.

  • Positive controlHeLa cell lysate, human tonsil tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab68265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP IP: 1/30.
IHC-P IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC ICC: 1/100 - 1/250.
  • Application notesIs unsuitable for or Flow Cyt.
  • Target

    • Alternative names
        Cdc2 related protein kinase antibodycdc2-related protein kinase antibodyCdk 2 antibody
        CDK2 antibodyCDK2_HUMAN antibodyCell devision kinase 2 antibodyCell division kinase 2 antibodyCell division protein kinase 2 antibodyCyclin dependent kinase 2 antibodycyclin dependent kinase 2-alpha antibodyCyclin-dependent kinase 2 antibodyp33 protein kinase antibodyp33(CDK2) antibody
      see all

    Anti-Cdk2 (phospho T14) antibody [EP2234Y] images

    • All lanes : Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/20000 dilution

      Lane 1 : HeLa cell lysate, untreated.
      Lane 2 : HeLa cell lysate, treated with hydroxyurea.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : ~34 kDa (why is the actual band size different from the predicted?)
    • Human tonsil tissue stained with ab68265 at 1/100-1/250 dilution.

    References for Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

    This product has been referenced in:
    • Li J  et al. Phosphorylation of MCM3 Protein by Cyclin E/Cyclin-dependent Kinase 2 (Cdk2) Regulates Its Function in Cell Cycle. J Biol Chem 286:39776-85 (2011). WB ; Human . Read more (PubMed: 21965652) »
    • Abdullah C  et al. Expression analysis and molecular targeting of cyclin-dependent kinases in advanced melanoma. Cell Cycle 10:977-88 (2011). WB ; Human . Read more (PubMed: 21358262) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"