• Product nameAnti-Cdk2 (phospho T14) antibody [EP2234Y]
    See all Cdk2 primary antibodies
  • Description
    Rabbit monoclonal [EP2234Y] to Cdk2 (phospho T14)
  • SpecificityThis antibody detects Cdk2 phosphorylated on Threonine 14.
  • Tested applicationsWB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human, Chinese Hamster

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.

  • Positive control
    • HeLa cell lysate, human tonsil tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab68265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP 1/30.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    Anti-Cdk2 (phospho T14) antibody [EP2234Y] images

    • All lanes : Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/20000 dilution

      Lane 1 : HeLa cell lysate, untreated.
      Lane 2 : HeLa cell lysate, treated with hydroxyurea.

      Lysates/proteins at 10 µg per lane.

      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : ~34 kDa (why is the actual band size different from the predicted?)
    • Human tonsil tissue stained with ab68265 at 1/100-1/250 dilution.

    References for Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

    This product has been referenced in:
    • Li J  et al. Phosphorylation of MCM3 Protein by Cyclin E/Cyclin-dependent Kinase 2 (Cdk2) Regulates Its Function in Cell Cycle. J Biol Chem 286:39776-85 (2011). WB ; Human . Read more (PubMed: 21965652) »
    • Abdullah C  et al. Expression analysis and molecular targeting of cyclin-dependent kinases in advanced melanoma. Cell Cycle 10:977-88 (2011). WB ; Human . Read more (PubMed: 21358262) »

    See all 3 Publications for this product

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