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Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

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Overview

Product name

Anti-Cdk2 (phospho T14) antibody [EP2234Y]
See all Cdk2 products (19) ...

Description

Rabbit monoclonal [EP2234Y] to Cdk2 (phospho T14)

Specificity

This antibody detects Cdk2 phosphorylated on Threonine 14.

Tested applications

WB, IP, IHC-P, ICCmore details

Cross reactivity

Reacts with

Human, Chinese Hamster

Does not react with

Mouse, Rat

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.

Positive control

HeLa cell lysate, human tonsil tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP2234Y

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

  • Western blot - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)Western blot - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab68265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000 - 1/20,000.De...Read more →

    WB: 1/5000 - 1/20,000.Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

  • IP

     IP: 1/30

    IP: 1/30

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.Per...Read more →

    IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

Application notes

Is unsuitable for or Flow Cyt.

Target

Function

Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.

Target information above from: UniProt accessionP24941 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdc2 related protein kinase antibody
  • Cdk 2 antibody
  • CDK2 antibody
  • CDK2_HUMAN antibody
  • Cell division kinase 2 antibody
  • Cell division protein kinase 2 antibody
  • Cyclin dependent kinase 2 antibody
  • Cyclin-dependent kinase 2 antibody
  • p33 protein kinase antibody
  • p33(CDK2) antibody
see all

Database links

Anti-Cdk2 (phospho T14) antibody [EP2234Y] images:

  Western blot - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

Western blot - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

All lanes : Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/20000 dilution

Lane 1 : HeLa cell lysate, untreated.
Lane 2 : HeLa cell lysate, treated with hydroxyurea.

Lysates/proteins at 10 µg per lane.

Secondary
HRP conjugated Goat anti-rabbit at 1/2000 dilution

Predicted band size : 34 kDa
Observed band size : ~34 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

Human tonsil tissue stained with ab68265 at 1/100-1/250 dilution.

References for Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265)

This product has been referenced in:

  • Li Jet al. Phosphorylation of MCM3 Protein by Cyclin E/Cyclin-dependent Kinase 2 (Cdk2) Regulates Its Function in Cell Cycle. J Biol Chem 286:39776-85 (2011). WB; Human.Read more (PubMed: 21965652) »
  • Prosser SLet al. Molecular dissection of the centrosome overduplication pathway in S-phase-arrested cells. Mol Cell Biol 29:1760-73 (2009). WB; Chinese Hamster.Read more (PubMed: 19139275) »

See all 2 publications for this product

Publishing research using ab68265? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"