Anti-Cdk2 (phospho T160) antibody (ab47330)
Overview
- Product nameAnti-Cdk2 (phospho T160) antibodySee all Cdk2 primary antibodies ...
- DescriptionRabbit polyclonal to Cdk2 (phospho T160)
- Tested applicationsWB, IHC-P, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of Threonine 160(T-Y-TP-H-E).
- Positive controlA2780 and MDA-MB-435 cell extracts, human breast carcinoma tissue.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Primary antibody notes This antibody detects CDK2 only when phosphorylated at Threonine 160.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab47330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| ELISA | ELISA: 1/4000. |
Target
- FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
- Unigene: 689624 Human
- Unigene: 111326 Mouse
- Unigene: 104460 Rat
see all
Target information above from: UniProt accession
P24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cdc2 related protein kinase antibodycdc2-related protein kinase antibodyCdk 2 antibody
- CDK2 antibodyCDK2_HUMAN antibodyCell devision kinase 2 antibodyCell division kinase 2 antibodyCell division protein kinase 2 antibodyCyclin dependent kinase 2 antibodycyclin dependent kinase 2-alpha antibodyCyclin-dependent kinase 2 antibodyp33 protein kinase antibodyp33(CDK2) antibody
see all
Anti-Cdk2 (phospho T160) antibody images
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Immunohistochemistry (Paraffin-embedded sections) - Cdk2 (phospho T160) antibody (ab47330)ab47330 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide. -
Western blot - Cdk2 (phospho T160) antibody (ab47330)All lanes : Anti-Cdk2 (phospho T160) antibody (ab47330) at 1/500 dilution
Lane 1 : A2780 cell extract preincubated with immunogenic phospho-peptide (1ug/ml)
Lane 2 : A2780 cell extract
Lane 3 : MDA-MB-435 cell extract
Lysates/proteins at 30 µg per lane.
Secondary
Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 0.12 µg/ml
Predicted band size : 34 kDa
Observed band size : 34 kDa
References for Anti-Cdk2 (phospho T160) antibody (ab47330)
This product has been referenced in:
- Ramathal C et al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol 30:1607-19 (2010). WB, IHC-P ; Mouse . Read more (PubMed: 20086097) »
- Cruse G et al. A novel FceRIß-chain truncation regulates human mast cell proliferation and survival. FASEB J 24:4047-57 (2010). WB ; Human . Read more (PubMed: 20554927) »
