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Immunohistochemistry (Paraffin-embedded sections) - Cdk2 (phospho T160) antibody (ab47330)
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Western blot - Cdk2 (phospho T160) antibody (ab47330)
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Product Name
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Cdk2 (phospho T160) antibody
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See all Cdk2 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdk2 (phospho T160)
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Immunogen
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Synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of Threonine 160(T-Y-TP-H-E).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/4000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A2780 and MDA-MB-435 cell extracts, human breast carcinoma tissue.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Relevance
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Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
This antibody detects CDK2 only when phosphorylated at Threonine 160.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk2 (phospho T160) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cdk2 (phospho T160) antibody images, references, products related to ab47330 and other tools.
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Cdk2 (phospho T160) antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Cdk2 (phospho T160) antibody (ab47330)
This image shows human breast carcinoma tissue stained with ab47330 at 1/50 dilution. Right hand image:tissue was treated with immunogenic peptide, left hand image: untreated.
ab47330 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide.
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Western blot - Cdk2 (phospho T160) antibody (ab47330)
All lanes : Cdk2 (phospho T160) antibody (ab47330) at 1/500 dilution
Lane 1 : A2780 cell extract preincubated with immunogenic phospho-peptide (1ug/ml)
Lane 2 : A2780 cell extract
Lane 3 : MDA-MB-435 cell extract
Lysates/proteins at 30 µg per lane.
Secondary
Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 0.12 µg/ml
Predicted band size : 34 kDa
Observed band size : 34 kDa
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Search PubMed (MEDLINE) for references to Cdk2
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Cdk2 (phospho T160) antibody - more information
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