Overview

  • Product nameAnti-Cdk2 (phospho T160) antibodySee all Cdk2 primary antibodies ...
  • Description
    Rabbit polyclonal to Cdk2 (phospho T160)
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of Threonine 160(T-Y-TP-H-E).

  • Positive control
    • A2780 and MDA-MB-435 cell extracts, human breast carcinoma tissue.
  • General notes


    This antibody detects CDK2 only when phosphorylated at Threonine 160.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Primary antibody notes This antibody detects CDK2 only when phosphorylated at Threonine 160.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab47330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent dilution.
ELISA 1/4000.

Target

Anti-Cdk2 (phospho T160) antibody images

  • ab47330 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide.
  • All lanes : Anti-Cdk2 (phospho T160) antibody (ab47330) at 1/500 dilution

    Lane 1 : A2780 cell extract preincubated with immunogenic phospho-peptide (1ug/ml)
    Lane 2 : A2780 cell extract
    Lane 3 : MDA-MB-435 cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 0.12 µg/ml

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References for Anti-Cdk2 (phospho T160) antibody (ab47330)

This product has been referenced in:
  • Ramathal C  et al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol 30:1607-19 (2010). WB, IHC-P ; Mouse . Read more (PubMed: 20086097) »
  • Cruse G  et al. A novel FceRIß-chain truncation regulates human mast cell proliferation and survival. FASEB J 24:4047-57 (2010). WB ; Human . Read more (PubMed: 20554927) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"