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Anti-Cdk2 (phospho T160) antibody (ab47330)

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Overview

Product name

Anti-Cdk2 (phospho T160) antibody
See all Cdk2 products (19) ...

Description

Rabbit polyclonal to Cdk2 (phospho T160)

Tested applications

WB, IHC-P, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of Threonine 160(T-Y-TP-H-E).

Positive control

A2780 and MDA-MB-435 cell extracts, human breast carcinoma tissue.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Primary antibody notes

This antibody detects CDK2 only when phosphorylated at Threonine 160.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

  • Immunohistochemistry (Paraffin-embedded sections) - Cdk2 (phospho T160) antibody (ab47330)Immunohistochemistry (Paraffin-embedded sections) - Cdk2 (phospho T160) antibody (ab47330) image (enlarge)

  • Western blot - Cdk2 (phospho T160) antibody (ab47330)Western blot - Cdk2 (phospho T160) antibody (ab47330) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab47330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/4000

    ELISA: 1/4000

Target

Function

Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.

Target information above from: UniProt accessionP24941 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdc2 related protein kinase antibody
  • Cdk 2 antibody
  • CDK2 antibody
  • CDK2_HUMAN antibody
  • Cell division kinase 2 antibody
  • Cell division protein kinase 2 antibody
  • Cyclin dependent kinase 2 antibody
  • Cyclin-dependent kinase 2 antibody
  • p33 protein kinase antibody
  • p33(CDK2) antibody
see all

Database links

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Anti-Cdk2 (phospho T160) antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - Cdk2 (phospho T160) antibody (ab47330)

Immunohistochemistry (Paraffin-embedded sections) - Cdk2 (phospho T160) antibody (ab47330)

ab47330 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide.

  Western blot - Cdk2 (phospho T160) antibody (ab47330)

Western blot - Cdk2 (phospho T160) antibody (ab47330)

All lanes : Anti-Cdk2 (phospho T160) antibody (ab47330) at 1/500 dilution

Lane 1 : A2780 cell extract preincubated with immunogenic phospho-peptide (1ug/ml)
Lane 2 : A2780 cell extract
Lane 3 : MDA-MB-435 cell extract

Lysates/proteins at 30 µg per lane.

Secondary
Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 0.12 µg/ml

Predicted band size : 34 kDa
Observed band size : 34 kDa

References for Anti-Cdk2 (phospho T160) antibody (ab47330)

This product has been referenced in:

  • Ramathal Cet al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol 30:1607-19 (2010). WB, IHC-P; Mouse.Read more (PubMed: 20086097) »
  • Cruse Get al. A novel FceRIß-chain truncation regulates human mast cell proliferation and survival. FASEB J 24:4047-57 (2010). WB; Human.Read more (PubMed: 20554927) »

See all 2 publications for this product

Publishing research using ab47330? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"