Overview

  • Product nameAnti-Cdk2 (phospho T39) antibodySee all Cdk2 primary antibodies ...
  • Description
    Rabbit polyclonal to Cdk2 (phospho T39)
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Zebrafish, Goldfish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cdk2.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: A431; Jurkat; HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab79379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

Target

Anti-Cdk2 (phospho T39) antibody images

  • All lanes : Anti-Cdk2 (phospho T39) antibody (ab79379) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysat with Immunising peptide at 1 µg/ml
    Lane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 10 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 11 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 12 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • ICC/IF image of ab79379 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79379 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol (100%, 5 minutes) fixed HepG2 cells at 10ug/ml

References for Anti-Cdk2 (phospho T39) antibody (ab79379)

ab79379 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"