Overview

  • Product name
    Anti-Cdk2 (phospho T39) antibody
    See all Cdk2 primary antibodies
  • Description
    Rabbit polyclonal to Cdk2 (phospho T39)
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Zebrafish, Goldfish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cdk2.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: A431; Jurkat; HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab79379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

Target

  • Function
    Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc2 related protein kinase antibody
    • cdc2-related protein kinase antibody
    • CDC28 antibody
    • CDC2A antibody
    • Cdk 2 antibody
    • CDK1 antibody
    • CDK2 antibody
    • CDK2_HUMAN antibody
    • CDKN2 antibody
    • Cell devision kinase 2 antibody
    • Cell division protein kinase 2 antibody
    • Cyclin dependent kinase 2 antibody
    • cyclin dependent kinase 2-alpha antibody
    • Cyclin-dependent kinase 2 antibody
    • kinase Cdc2 antibody
    • MPF antibody
    • p33 protein kinase antibody
    • p33(CDK2) antibody
    see all

Images

  • All lanes : Anti-Cdk2 (phospho T39) antibody (ab79379) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysat with Immunising peptide at 1 µg/ml
    Lane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 10 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 11 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
    Lane 12 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • ICC/IF image of ab79379 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79379 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol (100%, 5 minutes) fixed HepG2 cells at 10ug/ml

References

ab79379 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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