RabMAb

Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146)

Overview

  • Product nameAnti-Cdk2 (phospho Y15) antibody [EPR2233Y]
    See all Cdk2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2233Y] to Cdk2 (phospho Y15)
  • Specificityab76146 detects Cdk2 phosphorylated on tyrosine 15.
  • Tested applicationsIHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cdk2 (phospho Y15).

  • Positive control
    • WB: HeLa cell and mouse spleen tissue lysates. IHC-P: Human transitional cell carcinoma of bladder and rat colon tissues. IP: HeLa cell lysates.
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

WB 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/10000.

IP 1/100.

For unpurified use at 1/50.

  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] images

    • All lanes : Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/10000 dilution

      Lane 1 : Untreated HeLa cell lysate
      Lane 2 : HeLa cells treated with alkaline phosphatase lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1000

      Predicted band size : 34 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/10000 dilution (purified) + Mouse spleen tissue lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat ant-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 34 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/50000 dilution (unpurified)

      Lane 1 : HeLa cell lysates treated with LP
      Lane 2 : HeLa cell lysates untreated

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue labelling Cdk2 (phospho Y15) with purified ab76146 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. A prediluted HRP polymer-conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counter stained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Cdk2 (phospho Y15) with purified ab76146 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. A prediluted HRP polymer-conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counter stained with hematoxylin.

    • ab76146 (purified) at 1/100 immunoprecipitating Cdk2 (phospho Y15) in HeLa cell lysates (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References for Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146)

    This product has been referenced in:

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (10 %)
    Sample Human Cell lysate - whole cell (Hela cells)
    Specification Hela cells
    Treatment 2 ´M MK-1775
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Remi Buisson

    Verified customer

    Submitted Oct 27 2014

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (osteosarcoma cells)
    Specification osteosarcoma cells
    Treatment lane 1 control, lane 2 siRNA for CDK2, 48h
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    Submitted Sep 26 2014

    Thank you for your inquiry. I have confirmed with the laboratory that we have not tested cross-reactivity with phospho-CDK1. Sequence analysis shows that the immunogen of ab76146 shares 100% identity with human CDK1. Thus, I would predict cross-reac...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"