Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146)

Product Name 

Cdk2 (phospho Y15) antibody [EPR2233Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EPR2233Y] to Cdk2 (phospho Y15)

Immunogen 

A synthetic phospho-peptide corresponding to residues surrounding tyrosine 15 of human Cdk2

Reacts with 

Hu, Ms

Does not react with 
(species key)

Rat

Specificity 

ab76146 detects Cdk2 phosphorylated on tyrosine 15.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: 1/50.
WB: 1/5000 - 1/20000. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).

Is unsuitable for Flow Cyt or IHC-P.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysates.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

Relevance 

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EPR2233Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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