![Western blot - Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) image](#ab76146.jpg)
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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Overview
Product name
Anti-Cdk2 (phospho Y15) antibody [EPR2233Y]
See all Cdk2 products (19) ...
Description
Rabbit monoclonal [EPR2233Y] to Cdk2 (phospho Y15)
Specificity
ab76146 detects Cdk2 phosphorylated on tyrosine 15.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding tyrosine 15 of human Cdk2
Positive control
HeLa cell lysates.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2233Y
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Western blot - Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146)
![Western blot - Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) image](/ps/datasheet/Images/76/ab76146/ab76146.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab76146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/20000.Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).
IP
IP: 1/50IP: 1/50
Application notes
Is unsuitable for ,Flow Cyt or IHC-P.
Target
Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionP24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc2 related protein kinase antibody
- Cdk 2 antibody
- CDK2 antibody
see all
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- Unigene: 19192 Human
- Unigene: 689624 Human
- Unigene: 111326 Mouse
Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] images:
Western blot - Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146)
All lanes : Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/50000 dilution
Lane 1 : HeLa cell lysates treated with LP
Lane 2 : HeLa cell lysates untreated
Lysates/proteins at 10 µg per lane.
Predicted band size : 34 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
References for Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146)
This product has been referenced in:
- Kang Ket al. A Novel Topoisomerase Inhibitor, Daurinol, Suppresses Growth of HCT116 Cells with Low Hematological Toxicity Compared to Etoposide. Neoplasia 13:1043-57 (2011). WB; Human.Read more (PubMed: 22131880) »
- Liu Het al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB; Human.Read more (PubMed: 20818375) »
See all 2 publications for this product
Publishing research using ab76146? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"