Anti-Cdk4 antibody [AF10A4] (ab75511)

Overview

  • Product nameAnti-Cdk4 antibody [AF10A4]See all Cdk4 primary antibodies ...
  • Description
    Mouse monoclonal [AF10A4] to Cdk4
  • Tested applicationsSandwich ELISA, WB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Cdk4 protein (Human) purified from E.coli.

  • Positive control
    • Ramos, SK-N-MC, MCF-7, Jurkat, PC12 and NIH3T3 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab75511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA 1/200. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cdk4 (ab7955). For sandwich ELISA, use this antibody as Capture at 1/200 dilution with Rabbit polyclonal to Cdk4 (ab7955) as Detection.
WB 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionSer/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Involvement in diseaseDefects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
  • Cellular localizationCytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
  • Target information above from: UniProt accession P11802 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Cdk 4 antibody
    • cdk4 antibody
    • CDK4 protein antibody
    • CDK4_HUMAN antibody
    • Cell division kinase 4 antibody
    • Cell division protein kinase 4 antibody
    • CMM 3 antibody
    • CMM3 antibody
    • Crk3 antibody
    • Cyclin dependent kinase 4 antibody
    • Cyclin-dependent kinase 4 antibody
    • Melanoma cutaneous malignant 3 antibody
    • MGC14458 antibody
    • p34 cdk4 antibody
    • PSK J3 antibody
    • PSK-J3 antibody
    see all

Anti-Cdk4 antibody [AF10A4] images

  • Standard Curve for Cdk4 (Analyte: Cdk4 protein (T7 Tag) (ab40586)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AF10A4] to Cdk4 (ab75511) at 1/200 dilution. and Detector Antibody Rabbit polyclonal to Cdk4 (ab7955) at 0.5µg/ml.
  • All lanes : Anti-Cdk4 antibody [AF10A4] (ab75511) at 1/2000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : SK-N-MC cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH3T3 cell lysate


    Predicted band size : 34 kDa
    Observed band size : 34 kDa

Protocols

References for Anti-Cdk4 antibody [AF10A4] (ab75511)

ab75511 has not yet been referenced specifically in any publications.

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