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Synthetic peptide corresponding to Human Cdk4 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). synthetic peptide between aa 273-303
Database link: P11802
Our Abpromise guarantee covers the use of ab72090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/100 - 1/500. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).|
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Fluorescent image of Hela cell stained with ab72090. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab72090 antibody at 1/25 for 1 h at 37℃. For secondary antibody, Alexa Fluor®488 conjugated donkey anti-rabbit antibody (green) was used 1/400 dilution for 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor®555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).Cdk4 immunoreactivity is localized to Cytoplasm significantly.
ab72090 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"