You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Cdk4 antibody [DCS-31] - BSA and Azide free (ab77672)

Submit an Abreview Submit a Question

Overview

  • Product nameAnti-Cdk4 antibody [DCS-31] - BSA and Azide freeSee all Cdk4 primary antibodies ...
  • Description
    Mouse monoclonal [DCS-31] to Cdk4 - BSA and Azide free
  • Tested applicationsWB, IP, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human Cdk4 protein.

  • Epitopeaa 16-35
  • Positive controlMAD109, LS174T or PC12 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab77672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IP (Native and denatured): Use at 2µg/mg of lysate.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 34 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionSer/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
    • Involvement in diseaseDefects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
    • Cellular localizationCytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

      • Target information above from: UniProt accession P11802 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        Cdk 4 antibodycdk4 antibodyCDK4 protein antibody
        CDK4_HUMAN antibodyCell division kinase 4 antibodyCell division protein kinase 4 antibodyCMM 3 antibodyCMM3 antibodyCrk3 antibodyCyclin dependent kinase 4 antibodyCyclin-dependent kinase 4 antibodyMelanoma cutaneous malignant 3 antibodyMGC14458 antibodyp34 cdk4 antibodyPSK J3 antibodyPSK-J3 antibody
      see all

    References for Anti-Cdk4 antibody [DCS-31] - BSA and Azide free (ab77672)

    ab77672 has not yet been referenced specifically in any publications.

    Publishing research using ab77672 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab77672 Submit a Question for ab77672

    There are currently no Abreviews or Questions for ab77672.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"