Overview

  • Product nameAnti-Cdk4 antibody [DCS-35]See all Cdk4 primary antibodies ...
  • Description
    Mouse monoclonal [DCS-35] to Cdk4
  • Tested applicationsICC/IF, IP, WB, Functional Studies more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Recombinant full length protein (Human)

  • EpitopeAmino acids 1 - 20.
  • Positive control
    • NIH 3T3, MAD109, LS174T, or MCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent dilution.
IP Use at 2 µg/mg of lysate. (with protein G). Native only. Co-precipitates cyclin Ds.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Functional Studies Use at an assay dependent dilution. Kinase assay.

Target

  • FunctionSer/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Involvement in diseaseDefects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
  • Cellular localizationCytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
  • Target information above from: UniProt accession P11802 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Cdk 4 antibody
    • cdk4 antibody
    • CDK4 protein antibody
    • CDK4_HUMAN antibody
    • Cell division kinase 4 antibody
    • Cell division protein kinase 4 antibody
    • CMM 3 antibody
    • CMM3 antibody
    • Crk3 antibody
    • Cyclin dependent kinase 4 antibody
    • Cyclin-dependent kinase 4 antibody
    • Melanoma cutaneous malignant 3 antibody
    • MGC14458 antibody
    • p34 cdk4 antibody
    • PSK J3 antibody
    • PSK-J3 antibody
    see all

Anti-Cdk4 antibody [DCS-35] images

  • All lanes : Anti-Cdk4 antibody [DCS-35] (ab3112) at 2 µg/ml

    Lane 1 : Magic Mw Marker
    Lane 2 : Whole cell lysate from primary cortical neurons

    Lysates/proteins at 27 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse Ig at 1/10000 dilution
    developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 34 kDa


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Elena Schwartz

    See Abreview

References for Anti-Cdk4 antibody [DCS-35] (ab3112)

This product has been referenced in:

See all 9 Publications for this product

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Notre catalogue contient plus de 70 000 références anticorpset nous ne pouvons malheureusement pas offrir, pour des raisons logistiques et de stockage, d'échantillon gratuit ou de petit volume pour des essais préliminaires. ...

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Thank you for your inquiry. I can confirm that ab3112 is sold in a concentration of 1mg/ml as stated on the datasheet. I can further also confirm that ab6315 has a concentration of no less than 10mg/ml. For the batch available a...

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Application Western blot
Sample Rat Cell lysate - whole cell (primary cortical neurons)
Loading amount 27 µg
Specification primary cortical neurons
Gel Running Conditions Non-reduced Denaturing (4-12% Bis-Tris-Gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Elena Schwartz

Verified customer

Submitted Oct 03 2008

Thank you for your enquiry. The immunogen is purified full-length recombinant human Cdk4. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"