• Product nameAnti-Cdk4 antibody
    See all Cdk4 primary antibodies
  • Description
    Rabbit polyclonal to Cdk4
  • SpecificityReacts with Cdk4
  • Tested applicationsIP, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Cdk4 (C terminal). The peptide used is within the 50 amino acids at the carboxy terminus of CDK4.
    (Peptide available as ab8010)

  • Positive control
    • HeLa and K-562



Our Abpromise guarantee covers the use of ab7955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.Can be blocked with Cdk4 peptide (ab8010).

Starting dilution: 1:200

Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AF10A4] to Cdk4 (ab75511). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AF10A4] to Cdk4 (ab75511) as Capture.


  • FunctionSer/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Involvement in diseaseDefects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
  • Cellular localizationCytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdk 4 antibody
    • cdk4 antibody
    • CDK4 protein antibody
    • CDK4_HUMAN antibody
    • Cell division kinase 4 antibody
    • Cell division protein kinase 4 antibody
    • CMM 3 antibody
    • CMM3 antibody
    • Crk3 antibody
    • Cyclin dependent kinase 4 antibody
    • Cyclin-dependent kinase 4 antibody
    • Melanoma cutaneous malignant 3 antibody
    • MGC14458 antibody
    • p34 cdk4 antibody
    • PSK J3 antibody
    • PSK-J3 antibody
    see all

Anti-Cdk4 antibody images

  • Predicted band size : 34 kDa

    Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)

    Lanes 2, 4 and 6: CDK4 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Green signal from target – ab7955 observed at 34 kDa

    Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab7955 was shown to specifically react with CDK4 when CDK4 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK4 knockout samples were subjected to SDS-PAGE. ab7955 and ab8226 (loading control to beta actin) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Standard Curve for Cdk4 (Analyte: Cdk4 protein (T7 Tag) (ab40586)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AF10A4] to Cdk4 (ab75511) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk4 (ab7955) at 0.5µg/ml.


References for Anti-Cdk4 antibody (ab7955)

This product has been referenced in:
  • Kanatsu-Shinohara M  et al. Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc Natl Acad Sci U S A 111:8826-31 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24879440) »
  • Silva AR  et al. Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease. PLoS One 9:e99897 (2014). IHC-P ; Human . Read more (PubMed: 24936870) »

See all 6 Publications for this product

Product Wall

We have not determined a concentration of ab7955 to use in IHC-P. This is up to the end user to determine depending on their experiment. We can however suggest to start at 5 ug/ml (i.e 1/40 dilution). Some of our customers have also had good resul...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil, placenta)
Specification tonsil, placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
Permeabilization No

Abcam user community

Verified customer

Submitted Nov 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon tumor)
Specification Colon tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO target retrieval solution
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 23 2010

This antibody has been successfully used on formalin-fixed paraffin embedded normal human skin