For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Synthetic peptide corresponding to Mouse Cdk4 (C terminal). The peptide used is within the 50 amino acids at the carboxy terminus of CDK4.
(Peptide available as
Our Abpromise guarantee covers the use of ab7955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.Can be blocked with Cdk4 peptide (ab8010).
Starting dilution: 1:200
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AF10A4] to Cdk4 (ab75511). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AF10A4] to Cdk4 (ab75511) as Capture.|
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: CDK4 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target – ab7955 observed at 34 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab7955 was shown to specifically react with CDK4 when CDK4 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK4 knockout samples were subjected to SDS-PAGE. ab7955 and ab8226 (loading control to beta actin) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"