|
|
|
|
Western blot - Cdk5 antibody [2G2] (ab28441)
![Western blot - Cdk5 antibody [2G2] (ab28441)](/ps/datasheet/images/ab28441_1.JPG)
(enlarge)
|
|
|
|
|
Product Name
|
Cdk5 antibody [2G2]
|
| |
See all Cdk5 antibodies (13)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [2G2] to Cdk5
|
|
Immunogen
|
His-tagged recombinant human CDK5 protein purified from E. coli
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Tested applications
(see key)
|
ELISA, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution.
IP: Use at a concentration of 2 µg/ml.
WB: 1/1000 - 1/2000. Predicted molecular weight: 33 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
SH-SY5Y cell lysate
|
|
Cellular localization
|
Cytoplasmic. In axonal growth cone with extension to the peripheral lamellipodia.
|
|
Research areas
|
Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
|
|
Relevance
|
Cyclin dependent kinase 5 (CdK5) is a member of the cyclindependent kinase family of serine/threonine kinases. It is present in numerous mammalian tissues including kidney, testes, and ovary. Its activity is detected almost exclusively in brain extracts. Neuronal and muscle cells contain the highest amount of this protein. Similar to other Cdks, monomeric Cdk5 displays no enzymatic activity, but Cdk5 is not activated by cyclins. Instead, the p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 interacts with D1 and D3 type G1 cyclins and can phosphorylate histone H1, TAU, MAP2 and NF-H and NF-M. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
2G2
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: 0.03% Proprietary Preservative
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride Material safety datasheet (MSDS) for this product:
Glycerol MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk5 antibody [2G2] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Cdk5 antibody [2G2] images, references, products related to ab28441 and other tools.
|
| |
Cdk5 antibody [2G2] images:
|
 Western blot - Cdk5 antibody [2G2] (ab28441)
All lanes : Cdk5 antibody [2G2] (ab28441) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : SK-N-SH cell lysate
Lane 3 : Mouse brain tissue
Predicted band size : 33 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to Cdk5
|
| |
Cdk5 antibody [2G2] - more information
|
Customer reviews (feedback) regarding Cdk5 antibody [2G2] |
Customer FAQs regarding Cdk5 antibody [2G2] |
Protocols for Cdk5 antibody [2G2] |
Price and availability of products related to Cdk5 antibody [2G2] |
| |
Cdk5 antibody [2G2] - related products:
|
|
|
| |
Cdk5 antibody [2G2] - other tools:
|
Contact Abcam with a Technical Enquiry about Cdk5 antibody [2G2] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - Cdk5 antibody [2G2] (ab28441)](/index.html?datasheet=28441#ab28441_1.JPG)
