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Anti-Cdk5 antibody [EP715Y] (ab40773)

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Overview

Product name

Anti-Cdk5 antibody [EP715Y]
See all Cdk5 products (15) ...

Description

Rabbit monoclonal [EP715Y] to Cdk5

Tested applications

WB, IHC-P, ICC, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in the C-terminus of human Cdk5

Positive control

HeLa cell lysate; human brain

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

EP715Y

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Cell division
Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

  • Western blot - Cdk5 antibody [EP715Y] (ab40773)Western blot - Cdk5 antibody [EP715Y] (ab40773) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Cdk5 antibody [EP715Y] (ab40773)Immunohistochemistry (Paraffin-embedded sections) - Cdk5 antibody [EP715Y] (ab40773) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab40773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

    Four stars (1 Abreview) WB: 1/2000 - 1/10000.Det...Read more →

    WB: 1/2000 - 1/10000.Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • Flow Cyt

     Flow Cyt: 1/30

    Flow Cyt: 1/30

  • IP

     IP: 1/50

    IP: 1/50

Target

Function

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.

Cellular localization

Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.

Target information above from: UniProt accessionQ00535 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdk 5 antibody
  • Cdk5 antibody
  • CDK5_HUMAN antibody
  • Cell division protein kinase 5 antibody
  • Crk6 antibody
  • Cyclin dependent kinase 5 antibody
  • Cyclin-dependent kinase 5 antibody
  • Protein kinase CDK5 splicing antibody
  • PSSALRE antibody
  • Serine threonine protein kinase PSSALRE antibody
  • Serine/threonine-protein kinase PSSALRE antibody
  • Tau protein kinase II catalytic subunit antibody
  • TPKII catalytic subunit antibody
see all

Database links

see all

Anti-Cdk5 antibody [EP715Y] images:

  Western blot - Cdk5 antibody [EP715Y] (ab40773)

Western blot - Cdk5 antibody [EP715Y] (ab40773)

Anti-Cdk5 antibody [EP715Y] (ab40773) at 1/10000 dilution + HeLa cell lysate

Predicted band size : 33 kDa
Observed band size : 33 kDa

  Immunohistochemistry (Paraffin-embedded sections) - Cdk5 antibody [EP715Y] (ab40773)

Immunohistochemistry (Paraffin-embedded sections) - Cdk5 antibody [EP715Y] (ab40773)

Paraffin-embedded human brain stained for Cdk5 with ab40773 at 1/100 dilution

References for Anti-Cdk5 antibody [EP715Y] (ab40773)

This product has been referenced in:

  • Eggers JPet al. Cyclin-dependent kinase 5 is amplified and overexpressed in pancreatic cancer and activated by mutant K-Ras. Clin Cancer Res 17:6140-50 (2011). IHC-P; Human.Read more (PubMed: 21825040) »
  • Coras Ret al. Low proliferation and differentiation capacities of adult hippocampal stem cells correlate with memory dysfunction in humans. Brain 133:3359-72 (2010).Read more (PubMed: 20719879) »

See all 2 publications for this product

Publishing research using ab40773? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"