Overview

  • Product name
  • Description
    Mouse polyclonal to Cdk5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length human Cdk5 protein (NP_004926.1)

  • Positive control
    • Rat brain tissue lysate. Cell lysate from transfected 293T cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdk 5 antibody
    • Cdk5 antibody
    • CDK5_HUMAN antibody
    • Cell division protein kinase 5 antibody
    • Crk6 antibody
    • Cyclin dependent kinase 5 antibody
    • Cyclin-dependent kinase 5 antibody
    • Protein kinase CDK5 splicing antibody
    • PSSALRE antibody
    • Serine threonine protein kinase PSSALRE antibody
    • Serine/threonine-protein kinase PSSALRE antibody
    • Tau protein kinase II catalytic subunit antibody
    • TPKII catalytic subunit antibody
    see all

Images

  • Anti-Cdk5 antibody (ab88837) at 1 µg/ml + rat brain tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • All lanes : Anti-Cdk5 antibody (ab88837) at 1 µg/ml

    Lane 1 : cell lysate from Cdk5 transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • ab88837 at 10 µg/ml staining Cdk5 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.

References

ab88837 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (293 cells)
Specification
293 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

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Verified customer

Submitted Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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