Cdk5 (phospho Y15) antibody (ab63550)

Product Name 

Cdk5 (phospho Y15) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Cdk5 (phospho Y15)

Immunogen 

Synthetic synthesized phosphopeptide derived from human Cdk5 around the phosphorylation site of tyrosine 15 (G-T-Y^P-G-T).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Detects endogenous levels of Cdk5 only when phosphorylated at tyrosine 15.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from COS7 or 293 cells

Cellular localization 

Cytoplasmic. In axonal growth cone with extension to the peripheral lamellipodia.

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

Relevance 

Cyclin dependent kinase 5 (CdK5) is a member of the cyclindependent kinase family of serine/threonine kinases. It is present in numerous mammalian tissues including kidney, testes, and ovary. Its activity is detected almost exclusively in brain extracts. Neuronal and muscle cells contain the highest amount of this protein. Similar to other Cdks, monomeric Cdk5 displays no enzymatic activity, but Cdk5 is not activated by cyclins. Instead, the p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 interacts with D1 and D3 type G1 cyclins and can phosphorylate histone H1, TAU, MAP2 and NF-H and NF-M. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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