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Anti-Cdk5 (phospho Y15) antibody (ab63550)

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Overview

Product name

Anti-Cdk5 (phospho Y15) antibody
See all Cdk5 products (15) ...

Description

Rabbit polyclonal to Cdk5 (phospho Y15)

Specificity

Detects endogenous levels of Cdk5 only when phosphorylated at tyrosine 15.

Tested applications

WB, ELISA, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat

Immunogen

Synthetic synthesized phosphopeptide derived from human Cdk5 around the phosphorylation site of tyrosine 15 (G-T-YP-G-T).

Positive control

Extracts from COS7 or 293 cells

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Cell division
Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks

  • Western blot - Cdk5 (phospho Y15) antibody (ab63550)Western blot - Cdk5 (phospho Y15) antibody (ab63550) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab63550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 21435383)

Target

Function

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.

Cellular localization

Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.

Target information above from: UniProt accessionQ00535 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cdk 5 antibody
  • Cdk5 antibody
  • CDK5_HUMAN antibody
  • Cell division protein kinase 5 antibody
  • Crk6 antibody
  • Cyclin dependent kinase 5 antibody
  • Cyclin-dependent kinase 5 antibody
  • Protein kinase CDK5 splicing antibody
  • PSSALRE antibody
  • Serine threonine protein kinase PSSALRE antibody
  • Serine/threonine-protein kinase PSSALRE antibody
  • Tau protein kinase II catalytic subunit antibody
  • TPKII catalytic subunit antibody
see all

Database links

see all

Anti-Cdk5 (phospho Y15) antibody images:

  Western blot - Cdk5 (phospho Y15) antibody (ab63550)

Western blot - Cdk5 (phospho Y15) antibody (ab63550)

All lanes : Anti-Cdk5 (phospho Y15) antibody (ab63550) at 1/500 dilution

Lane 1 : extracts from COS7 cells treated with EGF (200ng/ml, 30mins) at 30 µg
Lane 2 : extracts from 293 cells treated with H2O2 (100µM, 15mins)
Lane 3 : extracts from 293 cells treated with H2O2 (100µM, 15mins) with immunizing peptide


Predicted band size : 33 kDa
Observed band size : 33 kDa


The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the immunising peptide for the WB is 5-10 ug.

References for Anti-Cdk5 (phospho Y15) antibody (ab63550)

This product has been referenced in:

  • Hu JPet al. Valproate reduces tau phosphorylation via cyclin-dependent kinase 5 and glycogen synthase kinase 3 signaling pathways. Brain Res Bull 85:194-200 (2011). WB, ICC/IF; Human, Mouse.Read more (PubMed: 21435383) »

See 1 publication for this product

Publishing research using ab63550? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"