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Cdk6 293T Transfected Lysate - (positive control) (ab94291)

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Overview

  • Product nameCdk6 293T Transfected Lysate - (positive control)See all Cdk6 lysates ...
  • General notesab94291 is a 293T cell transfected lysate in which Human Cdk6 has been transiently over-expressed using a pCMV-Cdk6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

    • Applications

    Our Abpromise guarantee covers the use of ab94291 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Cdk6 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Cdk6 293T Transfected Lysate - (positive control) (ab94291)
      SDS-PAGE - Cdk6 293T Transfected Lysate - (positive control) (ab94291)
      ab94291 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Cdk6 293T Transfected Lysate - (positive control) (ab94291)
      Western blot - Cdk6 293T Transfected Lysate - (positive control) (ab94291)
      All lanes : Anti-Cdk6 antibody (ab54576) at 1/500 dilution

      Lane 1 : Cdk6 293T Transfected Lysate - (positive control) (ab94291)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Cdk6 293T Transfected Lysate - (positive control) (ab94291)

    ab94291 has not yet been referenced specifically in any publications.

    Publishing research using ab94291 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"