Anti-Cdk6 (phospho Y13) antibody (ab194871)
Key features and details
- Rabbit polyclonal to Cdk6 (phospho Y13)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdk6 (phospho Y13) antibody
See all Cdk6 primary antibodies -
Description
Rabbit polyclonal to Cdk6 (phospho Y13) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cdk6 (phospho Y13). The exact sequence is proprietary.
Database link: Q00534 -
Positive control
- 293 cell lysate; Human breast carcinoma tissue and HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 1021 Human
- Omim: 603368 Human
- SwissProt: Q00534 Human
- Unigene: 119882 Human
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Alternative names
- CDK 6 antibody
- CDK6 antibody
- CDK6_HUMAN antibody
see all
Images
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All lanes : Anti-Cdk6 (phospho Y13) antibody (ab194871) at 1/500 dilution
Lane 1 : Untreated 293 cell lysate
Lane 2 : 293 cell lysate treated with HU
Predicted band size: 37 kDa -
Immunofluorescent staining of methanol-fixed HeLa cells labeling Cdk6 (phospho Y13) using ab194871 at 1/100 dilution.
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Immunohistochemical staining of Human breast carcinoma tissue labeling Cdk6 (phospho Y13) using ab194871 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab194871 has been referenced in 2 publications.
- Shi X et al. Discovery of rafoxanide as a dual CDK4/6 inhibitor for the treatment of skin cancer. Oncol Rep 40:1592-1600 (2018). PubMed: 29956794
- Wang H et al. Inhibition of experimental small-cell and non-small-cell lung cancers by novel antagonists of growth hormone-releasing hormone. Int J Cancer 142:2394-2404 (2018). PubMed: 29435973