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Western blot - Cdk7 antibody (ab58392)
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Immunohistochemistry (Paraffin-embedded sections) - Cdk7 antibody (ab58392)
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Product Name
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Cdk7 antibody
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See all Cdk7 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdk7
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Immunogen
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Synthetic peptide derived from the internal sequence of CDK7 (Human)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293 cell extract (Western blot). Human lung carcinoma tissue (IHC-P).
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Relevance
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Cdk7 is a member of the cyclin dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. Cdk7 forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk7 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cdk7 antibody images, references, products related to ab58392 and other tools.
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Cdk7 antibody images:
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 Western blot - Cdk7 antibody (ab58392)
All lanes : Cdk7 antibody (ab58392) at 1/500 dilution
Lane 1 : 293 cell extract with no immunizing peptide
Lane 2 : 293 cell extract with immunizing peptide
Predicted band size : 39 kDa
Observed band size : 41 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Paraffin-embedded sections) - Cdk7 antibody (ab58392)
ab58392, at 1/50 diltuion, staining human Cdk7 in lung carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin embedded tissue.
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Search PubMed (MEDLINE) for references to Cdk7
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Cdk7 antibody - more information
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Customer reviews (feedback) regarding Cdk7 antibody |
Customer FAQs regarding Cdk7 antibody |
Protocols for Cdk7 antibody |
Price and availability of products related to Cdk7 antibody |
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Cdk7 antibody - related products:
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Cdk7 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Cdk7 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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