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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Overview
Product name
Anti-Cdk7 antibody
See all Cdk7 products (11) ...
Description
Rabbit polyclonal to Cdk7
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Dog, Pig
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 330-346 of Human Cdk7.
Positive control
NIH3T3 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, Sucrose
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Applications
Show applications key
Our Abpromise guarantee covers the use of ab894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 36 kDa (predicted molecular weight: 41.5 kDa).
Target
Function
Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP50613
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 39 KDa protein kinase antibody
- CAK antibody
- CAK1 antibody
see all
Database links
- Entrez Gene: 1022 Human
- Entrez Gene: 12572 Mouse
- Omim: 601955 Human
- SwissProt: P50613 Human
- SwissProt: Q03147 Mouse
- Unigene: 184298 Human
- Unigene: 259718 Mouse
Anti-Cdk7 antibody images:
Western blot - Cdk7 antibody (ab894)
Predicted band size : 41.5 kDa
ab894 at 1/1000 dilution staining approximately 36kDa band of mouse cdk7 in NIH3T3 whole cell lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk7 antibody (ab894)
ab894 staining human epithelial cells of intestinal villus at 4.0 µg/ml by IHC-P.
References for Anti-Cdk7 antibody (ab894)
ab894 has not yet been referenced specifically in any publications.
Publishing research using ab894? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"