Recombinant Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6650(2)] to Cdk7 (phospho T170)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdk7 (phospho T170) antibody [EPR6650(2)]
See all Cdk7 primary antibodies -
Description
Rabbit monoclonal [EPR6650(2)] to Cdk7 (phospho T170) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A431, NIH/3T3 and C6 cell lysates and rat spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6650(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 39 kDa.
For unpurified use at 1/1000 - 1/10000. |
Notes |
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WB
1/1000. Predicted molecular weight: 39 kDa. For unpurified use at 1/1000 - 1/10000. |
Target
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Function
Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1022 Human
- Entrez Gene: 12572 Mouse
- Entrez Gene: 171150 Rat
- Omim: 601955 Human
- SwissProt: P50613 Human
- SwissProt: Q03147 Mouse
- SwissProt: P51952 Rat
- Unigene: 184298 Human
see all -
Alternative names
- 39 kDa protein kinase antibody
- CAK antibody
- CAK1 antibody
see all
Images
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All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + A431 cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + NIH/3T3 cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)
Lane 1 : C6 cell lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155976 has been referenced in 2 publications.
- Seoane M et al. Lineage-specific control of TFIIH by MITF determines transcriptional homeostasis and DNA repair. Oncogene 38:3616-3635 (2019). PubMed: 30651597
- Ghezzi C et al. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Nat Commun 10:5444 (2019). PubMed: 31784510