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Read our guarantee »Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Anti-Cdk7 (phospho T170) antibody
See all Cdk7 products (11) ...
Rabbit polyclonal to Cdk7 (phospho T170)
WB, IHC-P, ELISAmore details
Reacts with
Human
Synthetic peptide corresponding to amino acid residues surrounding Threonine 170 of human CDK7, conjugated to KLH
Ramos tissue lysate; Human breast carcinoma tissue
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration information loading...
Protein G purified
This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Our Abpromise guarantee covers the use of ab59987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P: 1/50 - 1/100.
ELISA: 1/1000
Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Ubiquitous.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity.
Nucleus.
Target information above from: UniProt accessionP50613
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Cdk7 (phospho T170) antibody (ab59987)

Anti-Cdk7 (phospho T170) antibody (ab59987) at 1/100 dilution + Ramos tissue lysate
Predicted band size : 39 kDa
Observed band size : 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk7 (phospho T170) antibody (ab59987)

Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab59987 at 1/50 dilution, followed by peroxidase conjugation to the secondary antibody and AEC staining.
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Anti-Cdk7 (phospho T170) antibody (ab59987) at 1/100 dilution + Ramos tissue lysate
Predicted band size : 39 kDa
Observed band size : 39 kDa

Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab59987 at 1/50 dilution, followed by peroxidase conjugation to the secondary antibody and AEC staining.
1
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