RabMAb

Anti-Cdk9 antibody [EPR3119] (ab76320)

Overview

  • Product nameAnti-Cdk9 antibody [EPR3119]See all Cdk9 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3119] to Cdk9
  • Tested applicationsWB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide from the C-terminus of Human Cdk9

  • Positive control
    • WB: HT-29, HL-60, HeLa or 293 cell lysates. IHC-P: Human breast carcinoma tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab76320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C 2k antibody
      • C-2K antibody
      • CDC2 related kinase antibody
      • CDC2L4 antibody
      • Cdk 9 antibody
      • Cdk9 antibody
      • CDK9_HUMAN antibody
      • Cell division cycle 2 like protein kinase 4 antibody
      • Cell division cycle 2-like protein kinase 4 antibody
      • Cell division protein kinase 9 antibody
      • CTK1 antibody
      • CTK1 antibody
      • Cyclin dependent kinase 9 antibody
      • Cyclin-dependent kinase 9 antibody
      • MGC95175 antibody
      • OTTHUMP0000002219 antibody
      • PITALRE antibody
      • RP11-228B15.5 antibody
      • Serine threonine protein kinase PITALRE antibody
      • Serine/threonine-protein kinase PITALRE antibody
      • TAK antibody
      • Tat associated kinase complex catalytic subunit antibody
      see all

    Anti-Cdk9 antibody [EPR3119] images

    • All lanes : Anti-Cdk9 antibody [EPR3119] (ab76320) at 1/2000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HL-60 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 43 kDa
    • ab76320 (1/200) staining Cdk9 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76320 at 1/100 dilution.
    • ab76320 showing positive staining in Cervical carcinoma tissue.

    • ab76320 showing positive staining in Colonic adenocarcinoma tissue.

    • ab76320 showing positive staining in Lung adenocarcinoma tissue.

    • ab76320 showing positive staining in Normal tonsil tissue.

    References for Anti-Cdk9 antibody [EPR3119] (ab76320)

    ab76320 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Oct 19 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
    Loading amount 0.5 µg
    Specification Xenopus laevis gastrula embryo extract (Stage12)
    Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Username

    Dr. Kei Miyamoto

    Verified customer

    Submitted Oct 10 2012

    Thank you very much for your interest in our products.

    We have three antibodies which may interest you as they are all tested for both IP and WB.In addition,the homology between the two species is 100% for this protein, therefore,it seems ver...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"