Anti-Cdk9 antibody [EPR3119] (ab76320)

Overview

• Product nameAnti-Cdk9 antibody [EPR3119]See all Cdk9 primary antibodies ...
• Description
  Rabbit monoclonal [EPR3119] to Cdk9
• Tested applicationsWB, IP, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide from the C-terminus of Human Cdk9

• Positive controlWB: HT-29, HL-60, HeLa or 293 cell lysates. IHC-P: Human breast carcinoma tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3119
• IsotypeIgG
• Research Areas
  • Microbiology
  • Protein
  • Human Protein
  • Defensin

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling
  • HIV-related

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P50750 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1025 Human
  • Entrez Gene: 1025 Human
  • Entrez Gene: 1025 Human
  • Omim: 603251 Human
  • SwissProt: P50750 Human
  • SwissProt: P50750 Human
  • SwissProt: P50750 Human
  • Unigene: 150423 Human

  • Unigene: 706809 Human

  see all

• Alternative names
  C 2k antibodyC-2K antibodyCDC2 related kinase antibody

  CDC2L4 antibodyCdk 9 antibodyCdk9 antibodyCDK9_HUMAN antibodyCell division cycle 2 like protein kinase 4 antibodyCell division cycle 2-like protein kinase 4 antibodyCell division protein kinase 9 antibodyCTK1 antibodyCTK1 antibodyCyclin dependent kinase 9 antibodyCyclin-dependent kinase 9 antibodyMGC95175 antibodyOTTHUMP0000002219 antibodyPITALRE antibodyRP11-228B15.5 antibodySerine threonine protein kinase PITALRE antibodySerine/threonine-protein kinase PITALRE antibodyTAK antibodyTat associated kinase complex catalytic subunit antibody

  see all

Anti-Cdk9 antibody [EPR3119] images

• 

  Western blot - Cdk9 antibody [EPR3119] (ab76320)
  All lanes : Anti-Cdk9 antibody [EPR3119] (ab76320) at 1/2000 dilution
  
  Lane 1 : HT-29 cell lysate
  Lane 2 : HL-60 cell lysate
  Lane 3 : HeLa cell lysate
  Lane 4 : 293 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-rabbit HRP at 1/1000 dilution
  
  Predicted band size : 43 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody [EPR3119] (ab76320)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
  ab76320 (1/200) staining Cdk9 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk9 antibody [EPR3119] (ab76320)
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76320 at 1/100 dilution.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119] (ab76320)
  ab76320 showing positive staining in Cervical carcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119] (ab76320)
  ab76320 showing positive staining in Colonic adenocarcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cdk9 antibody [EPR3119](ab76320)
  ab76320 showing positive staining in Lung adenocarcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119] (ab76320)
  ab76320 showing positive staining in Normal tonsil tissue.